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Trivia: During filming, Sarah Michelle Gellar became very close with Michael Gershman, the lead cinematographer of the first five seasons, who became a father figure to her (she was estranged from her real father). They became so close that when Gellar got married in 2002, she asked Gershman to dance with her during the father-daughter dance. When Gershman passed away in 2018, Gellar posted a heartfelt tribute to him on her social media.

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Act I - S1-E1

Continuity mistake: When Moist asks Dr. Horrible if he needs anything "dampened or made soggy?", the expression on his face changes instantly between cuts.

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Act I - S1-E1

Other mistake: During "My Freeze Ray", there's a shot where Billy, Penny, and several other people open doors to the laundry machines in sequence. When Billy opens the door to his machine, you can see the reflection of everyone else just standing there, waiting for their cue to walk forward.

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Act I - S1-E1

Continuity mistake: During the very beginning of "My Freeze Ray", as Dr. Horrible is about to set down his basket of laundry, the bottle of detergent sitting on top of his clothing rotates 90 degrees after the scene briefly cuts to Penny.

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Trivia: A sequel went into production after this film came out, with stars Melissa Barrera and Jenna Ortega returning, and Christopher Landon as director. However, in late 2023, all three left the project. Barrera was fired for pro-Palestine social media posts, which the studio claimed were antisemitic despite her stating they were not intended to be. Ortega then left over a pay dispute. Finally, Landon dropped out, calling the situation a "nightmare." (Presumably due to how actresses were treated.)

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Trivia: Zack Snyder originally intended for the movie to be an R-rated spin-off "Star Wars", but Lucasfilm wasn't interested in his ideas. It was eventually retooled into a stand-alone work.

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Trivia: Director Mark Steven Johnson admitted in a 2023 interview celebrating the film's 20th anniversary that his one big regret was not telling a smaller story. In retrospect, he feels that putting a Daredevil origin story, an Elektra story, court/law-themed story (in the Director's Cut) and a Kingpin story was a little too much for one movie, and some of the material should have been saved for a potential sequel. But as a Daredevil fan, he wanted to put in everything he loved about the comic.

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Trivia: While the logo was introduced a year prior in 2002's "Spider-Man", this is the first film in which the Marvel "flipping pages" logo actually had the flipping pages sound effect added. To this day, 20 years later, that logo and sound effect are still sometimes used in non-MCU Marvel films.

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Trivia: Despite already being very tall at 6'5", Michael Clarke Duncan was often standing on boxes or wearing lift shoes in many of his scenes to add several more inches and make him look almost inhumanly massive. Specific camera angles were also used to make him look larger.

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Trivia: Michael Clarke Duncan was so well-liked in the role of Kingpin that he was asked to reprise the role (in voice-over form) in the 2003 "Spider-Man" animated series on MTV.

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Trivia: Filmmaker and actor Harold Ramis wanted to direct the film as he was a big video game fan and especially a big "Super Mario" fan (even putting a reference to Mario in "Ghostbusters 2"). He took a meeting with the producers but ended up passing on the project, as he had a bad feeling about it.

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Revealing mistake: When Richter's arms get cut off and he falls down the elevator shaft to his death, you can very obviously see the outlines of his real arms inside his shirt when his leather jacket flies up as he falls.

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Continuity mistake: When Valentina grabs the wire in front of her neck and cuts her hands, the blood pattern changes between cuts. (There's suddenly less blood in the second shot, revealing it's a different take.)

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Continuity mistake: When Gabriella is kidnapped, as Amanda approaches the door of the stall she's in, the way she's holding her purse changes between shots. When it cuts to the angle above, the straps go from over her hands to under her hands.

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Visible crew/equipment: When John returns to the clinic to drop off a gift for Gabriele, when the cab pulls up, for a split second, you can see what appears to be the reflection of a crew member (likely part of the camera crew) walking in the green doors right before John opens his door. (It's very hard to see, but you can see it if you pay close attention.)

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Audio problem: When John wakes up after the surgery, his lines "Hemoglobin's normal" and "After that, what happens then?" are obviously dubbed in after the fact. His voice is much louder than he appears to be speaking, revealing that they were re-recorded during post-production.

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Continuity mistake: When John is fixing Carlos' bike wheel, throughout the scene, it is leaning backwards towards John. However, right before he pulls it out of the vice, it is suddenly leaning forwards towards Carlos. (John never pushed it forward.)

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Continuity mistake: When John puts Carlos' bike wheel in the vice, his right hand goes from cranking the vice to holding the side of the wheel as it cuts to Carlos.

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Continuity mistake: After Cecilia points out Carlos and his father to John while showing him around, they begin walking again. John goes from walking a few feet behind Cecilia to right next to her between cuts.

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Continuity mistake: When John is watching the video of Cecilia, he reaches up and puts his right hand against his face. It then cuts to the opposite angle, and suddenly it's switched to John's left hand being against his face.

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