Continuity mistake: After Mary Jane slips while carrying the food tray and the food is thrown into the air, the shot facing the ceiling showing the food falling shows the bowl of jello to hit first and the apple to hit last, while the next shot shows Peter catching the apple first and the bowl of jello last. (00:21:10)
Continuity mistake: When Peter is trying to talk to Mary in the cafeteria you can see behind where he is standing there are numerous people and tables between him and the back wall. Peter then sits down and in the very next shot his table is one table away from the wall behind him. The shot may be lower but is from the same angle as before. (00:21:35)
Continuity mistake: In the school cafeteria after Peter saves Mary Jane, he notices the fork that is stuck to his hand. If you look at the back of his hand, the spider bite is gone. In the next scene, it is back again. (00:21:45)
Continuity mistake: In the cafeteria scene, Mary Jane slips on some spilled orange juice that causes Peter to catch her. A short while later when Peter gets up to leave the cafeteria, running away from her enraged boyfriend, the spilled orange juice is not there. (00:22:15)
Continuity mistake: When Peter accidentally flings the tray of food on Flash it hits him on the left shoulder but when Flash stands up the stain temporarily jumps over to his right shoulder. (00:23:19)
Continuity mistake: In the fight with Flash Thompson (in school, his first fight), when Peter grabs his hand, first he is grabbing him by his watch, then a little bit lower in a different shot, in the next shot by his watch again. (00:23:40)
Continuity mistake: After Peter punches Flash in the guts during the Flash/Peter hallway fight scene, in Mary Jane's closeup she looks surprised, and behind her on her right is a surprised guy wearing glasses. After Flash's friend says "Jesus Parker, you are a freak," and Harry says "Wow, that was amazing", the same guy suddenly appears on Mary Jane's left, looking happy and clapping. It cuts to a closeup of Mary Jane and the same guy appears back on Mary Jane's right, looking somewhat surprised again. (00:23:50)
Continuity mistake: When Peter runs out of school just after he beat up Flash, he runs into an alley and looks at his hand which is at waist level, then it cuts to a different view, his hand is now at neck level and one of his fingers is pointing up, then the shot after his hand is back to waist level and all fingers are straight and pointing down. (00:24:20)
Continuity mistake: In the scene in which Peter and Flash are fighting in the hall, Harry runs up to Mary Jane to see what is happening, seconds later Peter jumps up to avoid Flash's friend charging him from behind. In the shot of Peter flipping in the air, you can see that Harry is no longer standing next to Mary Jane, a girl in a blue sweater is. After Peter lands, the camera cuts back to Mary Jane and Harry is once again by her side. (00:24:25)
Continuity mistake: When Peter Parker runs into the alley and tests his wall-crawling for the first time, when he runs into the alley the wall he climbs is brick covered with dirty black soot/fungus. After he begins his climb it is completely brown. (00:24:50)
Continuity mistake: Right after Peter has beats up Flash, only Mary-Jane stands to the right of Peter. If you look behind her there are two extras, to the right of her whenever there is a far shot, and to the left of her on the close up of her. (00:25:00)
Continuity mistake: When Peter first learns he can climb walls after running out of school, his shoes are different when he is on the wall compared to when he is on the roof. (00:26:20)
Continuity mistake: When Peter is taking out the trash, and MJ walks out and says, "Were you listening to that?". Watch Peter as he turns around to look at MJ twice. (00:27:40)
Continuity mistake: When Peter is taking out the trash, and begins talking to Mary Jane, as she walks toward him you can see her underwear sticking out above her pants. As the shots change, this underwear seems to appear and disappear. (00:28:40)
Continuity mistake: When MJ and Peter are discussing the beating that Peter gave Flash that morning in school, MJ says, "He's just glad you didn't give him a black eye for graduation." This scene takes place the day after Peter was bitten by the spider, when the weather is cold. High school graduations generally take place in June or late May - so any black eye Peter might have given Flash would long be healed up by graduation. (00:29:15)
Suggested correction: I think it's just figurative speech, exaggeration. He hits him on the chest after all.
Continuity mistake: When Peter is drawing up ideas for his costume the hand is that of comic artist Phil Jimenez, current artist of Wonder. Phil Jimenez is right handed and Tobey is left handed. In one of the cuts the pen is in Tobey's left hand but it shows him drawing with his right. (00:31:25)
Continuity mistake: When Peter shoots his web at his bedroom lamp and pulls it across the room, it smashes against the wall and breaks. But when Aunt May is talking to Peter from the door seconds later, the lamp is back on the dresser in one piece. (00:32:15)
Continuity mistake: When Peter and his uncle are talking in the car, they are parked on the side of a one way street, with their car in the same direction as the traffic, but when the camera is on the uncle, you can see cars going in the opposite direction reflected in the windows behind him. (00:33:10)
Continuity mistake: When Peter shoots webs around his room and hits the rocket over, all that is on the nightstand is the cool light bulb lamp, the picture and the Dr. Pepper can. When it flips back to the nightstand again you can see some weird green alarm clock next to the overturned picture. (00:33:15)
Continuity mistake: When Peter is practising throwing spider webs in his bedroom, his first attempt hits a model rocket and it breaks in many pieces. A few shots later when he finally hits the Dr. Pepper can, as he whips it across the room you can see the rocket back in one piece, just leaning against the wall. (00:33:15)