Continuity mistake: When Osborn awakens in the vault, the position of his hand grabbing the doctor's throat changes positions depending on the angle.
Continuity mistake: Bone Saw had four valets during the wrestling scene yet during the cage match he's only got three at ringside, one of the two blondes disappears.
Suggested correction: I just loaded up the scene. All four of them are definitely there during the cage match. There's even several shots where you can see all four at the same time.
Continuity mistake: When Spider-Man is being gassed by the Green Goblin, the mouthpiece of the Green Goblin's mask is open, and Osborne's lips are visible moving as he speaks to J. Jonah Jameson. Later in the scene where Spider-Man fights the Green Goblin in the burning building, a close-up of his mask is shown again, but this time there appears to be some kind of black mesh screen in the mouth of his mask.
Suggested correction: It's never fully open, it's just easier to see through the mesh in certain lighting.
Continuity mistake: This is a little hard to explain. During the Unity Day Festival, after Goblin beats up the cops, the shot cuts and he turns around to face Spider-Man, who lands in front of him. The problem? He was facing the same direction as Spider-Man in the previous shot (you can tell from the background), so it makes no sense that he has to turn around when the camera cuts.
Continuity mistake: At the Unity Day Festival, when Goblin says "Impressive!" and kicks Spider-Man away, watch closely. Goblin goes from standing on top of a bare ground to standing on top of some sort-of tarp instantly between cuts as Spider-Man hits the first table.
Deliberate mistake: During the fight at the Unity Day Festival, at one point Goblin is confronted by some cops and beats them up. Watch closely... you can tell that the first and second shots are just different takes of the same stunt from a different camera angle. Goblin's movies are exactly the same (except slightly faster in the second shot), and you can also tell it's the exact same two cops he's hitting. They tried to artificially lengthen the scene by showing two slightly different takes of the stunt.
Continuity mistake: During the World Unity Festival, after the balcony begins to collapse, Mary Jane hits the railing and a support falls from it. The support reappears and stays until she falls.
Audio problem: The wrestler's, wheeled out prior to Peter wrestling Bone Saw, mouth doesn't match the dialogue as he's speaking.
Continuity mistake: When Mary Jane falls off the balcony she falls sideways except for a shot of her falling face down. Shot changes and she is falling sideways again.
Continuity mistake: When Peter wakes up the day after he was bitten by the spider and goes to the living room doing a little "stunt", Uncle Ben immediately turns to him, but in the next shot he makes a forward movement as Peter falls on the ground after the stunt.
Continuity mistake: When Peter tries his first web swinging on the rooftop, the sky turns from sunny to cloudy between shots.
Continuity mistake: When Flash is hit by the tray with food he raises his arms in disbelief. A shot later his arms are lowered and he repeats the previous movements.
Continuity mistake: When the movie begins and all the students enter the lab, Mary Jane's boyfriend nags her and she moves her arm to her shoulder, then lowers it. In the following shot her arm is raised and she is lowering it again.
Continuity mistake: When Osborn is getting ready to radiate himself at his laboratory, he throws his clothes on the floor. Their position differs between the first and second shot.
Continuity mistake: The day after Peter gets his powers, while he follows Mary Jane to the school, the sun changes between shots.
Revealing mistake: When the car jacker who stole Uncle Ben's car is being chased by the police, they seem to be doing a high speed pursuit. However when the car goes over the corner and demolishes a stand, a cut shows the inside of the car. The digital speedometer is reading 15-17 mph, hardly much of a challenge for the police to catch.
Continuity mistake: When Peter is testing the web on the crane, his fringe swaps between messy/ brushed /windy / still between frames in no order whatsoever.
Continuity mistake: When Osborn is drinking whiskey and hears his inner voice, is about to see the African masks and before watching himself in the mirror, his fringe swaps from messy to brushed.
Continuity mistake: When Osborn is laid on the stretcher about to enter the vault, the cables by his right shoulder change positions between shots.
Continuity mistake: When Aunt May opens Peter's sleeve to show his wound, the opening differs between frames.