Plot hole: Pike says he doesn't want women on his bridge, Number One being the one and only exception, and he keeps ordering Colt off it. His apology to Number One indicates that A) he doesn't think of her as female, and B) she's the only exception to his rule. So how does he overlook the very female crew-woman seated at the science station? (00:06:55)
The Man Trap - S1-E2
Plot hole: The salt creature needs salt, but there is no need for the creature to kill anybody, just have them deliver a large shipment of salt the next time some ship come to visit the planet.
Suggested correction: As shown several times, the creature is acting on craving and impulse. It's able to not attack Dr. Crater because they have a mutual relationship, as well as he provides salt for her from their supplies. But their supplies are running low, as he even stated they did have 25 pounds of salt but displays a jar with barely any left in it. It is reasonable to assume that he has had to start rationing the supply of salt to her to keep her till they could get another shipment. They were not getting the salt right off after Kirk arrived, and so the creature could not resist the urge to suck salt it needed out of the crewman when they were together alone, reason and logic being clouded by its desire and feral cravings.
Plot hole: Because of the magnetic ore on the work clothes of the man who beamed up, the transporter made duplicates of everything put through it, and so couldn't be used. This was further complicated by the control and power circuits being blasted by the "evil" Kirk. However, the ship carries a number of shuttle craft, which no-one mentions.
Plot hole: 300 years is just too long for the children to be on their own. How did they keep their clothes relatively clean for 300 years? Since the kids are playing all day they aren't out in the fields planting and harvesting crops for food, how did they eat?
Suggested correction: For the clothes, it's highly unlikely that they wore the same sets for 300 years. They are in a town/city and on a world that is mostly empty of most human life now. They can easily just find more clothing their size from other houses and even stores. As far as food, children are very good scavengers.
The issue of clothing is not so much an issue as is the issue of food. Given that the children are growing at an incredibly slow rate, their metabolism is probably much, much, much slower and would require far less sustenance.
Plot hole: For a penal colony, Tantalus V has really poor security. Van Gelder manages to escape with ease, by hiding in a box - the only box - beamed up to the Enterprise.
Plot hole: Apparently there is some confusion over the distance between Earth, Starbase 11, and Talos IV. When Spock first meets Pike on Starbase 11 he tells Pike Talos IV is only six days away. Yet when Pike (in the recording) speaks to the Talosians for the first time, he says he is from a star system on the other side of the galaxy. If Talos IV was on one side of the galaxy and Earth was on the other side, it would take hundreds of years at maximum warp to travel from one planet to the other.
The Conscience of the King - S1-E14
Plot hole: After Kirk sees Leighton laying next to the rocks, he rushes over to examine him. He places one hand on Leighton's arm and the other on Leighton's waist and declares "It's Tom Leighton...he's dead." All this without the use of a medical tricorder or even the experienced opinion of the ship's doctor to confirm what Kirk is seeing. (00:10:46)
Shore Leave - S1-E16
Plot hole: The planet is supposed to be uninhabited but nobody seems surprised that there are walkways everywhere.
Tomorrow is Yesterday - S1-E20
Plot hole: In the transporter room the captain calls to see if Major Christopher is ready, Christopher somehow knows exactly what button to press on the console to answer back.
Space Seed - S1-E23
Plot hole: It is stated during the episode that Khan was, at one time, the ruler of over 1/4th the Earth's population, during a very key moment in the planet's history (The "Eugenics" Wars). Such a personage undoubtedly would be very well known to 23rd century Earth people, at a level of infamy approximating Julius Caeser or Adolf Hitler. Yet it is only 2/3rds the way through the episode, thanks to a computer search by Spock, that the crew divines his identity. Lt. McGivers at the very least should have almost instantly recognized him.
A Taste of Armageddon - S1-E24
Plot hole: The ambassador and his aide beam down to the planet. This would not be possible because earlier on Scotty refused to lower the screens/shields until the Captain told him to do so. It had already been established in an earlier episode titled "Arena" that transporters don't work with screens up. (00:32:40)
This Side of Paradise - S1-E25
Plot hole: When Captain Kirk is the last person unaffected by the spores, he's on the bridge lamenting the fact that he is trapped in orbit above the planet since he can't pilot the ship alone. He also can't call for help because Lt. Uhura sabotaged long range communications. but Captain Kirk has somehow forgotten about the shuttle craft. He could have easily escaped on one of those.
Plot hole: Spock has absolutely no way to know, yet, that the horta only secretes her corrosive substance when tunneling: he hasn't had time to examine her or to do more than determine that she does indeed secrete a substance that cuts the tunnels. So he should at the very least scan the piece of her that falls off before he picks it up with his bare hands. Major lapse of logic, which given his character goes beyond a character mistake.
The Alternative Factor - S1-E28
Plot hole: Kirk knows that Lazarus is insane and that he wants the Enterprise dilithium crystals. Yet he's not restrained in sickbay and is, in fact, given free run of the ship so that he can knock out the crew in engineering and steal the crystals. Other than to further a woefully weak plotline, this makes no sense whatsoever.
Plot hole: Commodore Decker takes over command of the enterprise but Spock says if McCoy can certify him incompetent (which he obviously is) he can be relieved of command. McCoy says he will certify him now, so why doesn't he? In other episodes it has been stated that McCoy can order anybody regardless of rank to an examination to see if they are physically or mentally fit.
Plot hole: How did the android Norman get aboard the enterprise? If he beamed aboard I'm sure someone would have noticed and where did he beam from? The Enterprise was nowhere near any planet and I'm sure they would have detected any spacecraft nearby.
Suggested correction: These are unanswered questions, not plot holes.
But something phrased as a question because it has no decent answer can constitute a plot hole.
The Ultimate Computer - S2-E24
Plot hole: When M-5 destroys the ore freighter, Dr. Daystrom says, "Fortunately, it was only a robot ship." But Daystrom wasn't on the bridge yet when Spock announced that fact. He didn't have any way of knowing that the freighter was unmanned. (00:23:00 - 00:25:05)
Plot hole: Scotty is receiving images of the launch, supposedly from an orbiting satellite. But many of the camera views are obviously from ground level, and couldn't possibly have been taken from orbit. It's not a rebroadcast from a ground camera, either. The implication is clearly that Scotty is is picking up a live satellite feed. (00:34:30)
Plot hole: Spock claims that history is unchanged at the end of the episode according to the library tapes - how would he know? If history changed, the tapes would change too. And unlike in "City on the Edge of Forever", there's no Guardian around to keep people from being influenced by an altered timeline.
The Enterprise Incident - S3-E2
Plot hole: In the conference room, Spock informs Kirk that his theory of a Romulan cloaking device kept the sensors from detecting the ships. However, in S1:E14, "Balance of Terror", they figured out the cloaking device, and how it worked. Unless he was feeding into the deception, Spock shouldn't have had any theory when it was a fact.