Continuity mistake: After Luigi tells Mario to let go of the Bob-omb in the Mushroom Land, his black sideburns briefly turn skin colour. (00:15:51)
Visible crew/equipment: In the live-action segment, several times when Mario and Luigi confront Mrs. Gammliss, the shadow of either a crew member or a piece of equipment is moving at the top of the screen. It is not Mario, Luigi, or the Ratagator's, as they are not moving, and it is not the passersby's, as the shadow is present even when they are not present. (00:17:38 - 00:18:45)
Revealing mistake: In the live-action segment, when the glass in Luigi's hand shatters from Mrs. Gammliss' singing, Luigi can be seen actually pressing the glass together to make it break instead. (00:17:59)
Raiders of the Lost Mushroom - S1-E49
Revealing mistake: When Indiana Joe saves Luigi, he is layered over a mushroom. (00:05:05)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: When Kolonel von Koop says that Mario and his friends are not going to have the Lost Mushroom, one of his white nails turns blue, like his shirt. (00:05:38)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: When Indiana Joe says that he will not enter the Temple of Koopa, his brown bag turns yellow. (00:06:46)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: Throughout its appearance, the Cobrat in the fishbowl has its eyes and underbelly changing colour several times. (00:08:11)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: When Kolonel von Koop approaches Mario and his friends, his tank top and belt disappear. (00:09:40)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: When Kolonel von Koop says that Mario and his friends are finished, the pink wristbands on Mouser's gloves turn white, like his shirt. (00:09:47)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: When Mouser and Koopa Troopa pull out their Cobrats, Mouser's band switches from his left arm to his right, while Kolonel von Koop's right arm changes from green to red. (00:09:54)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: When Mouser wonders if the Lost Mushroom can make a castle out of cheese, his grey trousers turn white like his shirt, while Koopa Troopa's yellow eyes turn white. (00:13:07)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: When Kolonel von Koop and his minions are surprised that Mario and his friends survived, Koopa Troopa's yellow face turns green like the rest of his body, while Mouser's skin-color tail turns grey like his trousers. (00:13:28)
Raiders of the Lost Mushroom - S1-E49
Audio problem: Shortly after Mario's friends arrive, Mario's mouth moves, but no sound can be heard. (00:13:48)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: Throughout most of the episode, Kolonel von Koop wears a tank top, but when he is surrounded by Mario's friends, it turns into a shirt. (00:15:02)
Raiders of the Lost Mushroom - S1-E49
Audio problem: When Mario picks up the Lost Mushroom for the first time, his mouth is moving, but not saying anything. (00:32:10)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: Near the end, when Koopa escapes through the warp you will see, for a split second, that Mario and Luigi have switched their colors. (00:38:15)
Raiders of the Lost Mushroom - S1-E49
Continuity mistake: When Koopa escapes through the warp at the end, the hole closes up. When Mario says "We sure whipped him this time," (meaning Koopa) look at the ground and you will see that everyone is standing next to the wide open hole. (00:38:25)
Continuity mistake: Throughout most of the episode, the mayor's mustache is brown, but in his initial appearance, it is black instead. (00:04:34)
Revealing mistake: When the Mushroomers are scared of the crocodiles entering the town, the crocodiles freeze for a few frames in the transition to the next shot. (00:05:04)
Continuity mistake: When Princess Toadstool says that one of the crocodiles wants to eat Toad, her left eyebrow disappears briefly. (00:05:18)