Revealing mistake: In the live-action segment, when E.C. arrives, the basement shakes but the objects remain stationary, revealing that the shaking was achieved by shaking the camera. (00:02:10)
Continuity mistake: Throughout its appearance, the grey sideskirts on Mario and his friends' car turn brown like the rest of the car several times. (00:03:44 - 00:09:06)
Continuity mistake: At the beginning of the episode, when Koopa's state Troopa makes the Mario gang pull over, both vehicles stop in the middle of the road. From there on, the cars are at the side of the road. (00:13:55 - 00:16:25)
Continuity mistake: When Mario, Luigi and Toad say goodbye to the Princess, Toad is standing at the front of their car, Luigi in the middle, and Mario at the back. Two shots later, the order changes to Mario at the front, then Toad, and Luigi at the back of the car. (00:15:10)
Continuity mistake: After the Marios say goodbye to the Princess, Mario is at the front of their car, then Toad, and Luigi at the back. Mario and Luigi swap places for the rest of the scene. (00:15:15 - 00:16:10)
Continuity mistake: When Toad screams and runs away after the Bob-omb lights, Mario, Luigi and their car disappear. (00:16:00)
Continuity mistake: As Mario, Luigi and Toad speed to the rebel fort, the side-view mirrors keep disappearing. (00:17:25 - 00:18:40)
Continuity mistake: When Mario, Toad and Luigi are driving to the rebel fort, the seatbelts they are wearing disappear for most of the scene. (00:17:30 - 00:18:40)
Continuity mistake: When the Mario Bros. speed up their car to get to the rebel fort, in one shot both of Mario's hands are on the steering wheel, and in the next shot his right hand is on some sort of lever that makes the car go faster. Luigi's hands also change position in these shots. (00:17:45)
Continuity mistake: When Koopa sends Mouser to hunt down the Marios after finding out they've escaped, the binoculars that he was holding have disappeared. (00:17:55)
Other mistake: When Mario, Luigi and Toad are speeding to get to the rebel fort, Toad tells Mario to look out the car's rear-view mirror. However, the car doesn't have one. (00:18:15)
Other mistake: Koopa's army tries to stop the Marios from getting to the rebel fort by driving a truck and stopping it in the middle of the road. However, nobody is driving the truck. (00:18:25)
Continuity mistake: The Thunderbirdo is supposed to be pink. It is green when first visible after Koopa orders it up. It turns green again right before it explodes. (00:19:15 - 00:23:55)
Continuity mistake: When the ketchup lorry crosses the bridge, it has a cream cab and a red trailer, but in the next shot, it has a blue cab and a grey trailer.
Continuity mistake: After Princess Toadstool is captured by Blackbeard Koopa, the Koopa Troopa holding her left arm has a blue bandanna. But when Blackbeard Koopa says that she did not come willingly, the bandanna turns red. (00:07:53)
Continuity mistake: When Captain Clump says that Blackbeard Koopa bothers pirates, his purple trousers turn red. (00:07:59)
Continuity mistake: Normally, Blackbeard Koopa's ship is blue, but when Princess Toadstool is on his ship, it turns brown like Captain Clump's. This also happens when it is docked at his fortress. (00:08:29 - 00:11:17)
Continuity mistake: When the Mario Bros. are revealed, Mario is wearing the tank top of his disguise, but in the next shot, he is wearing his normal clothes. (00:15:18)
Two Plumbers and a Baby - S1-E17
Continuity mistake: Normally, the castle has a door, but when King Koopa says that the infants will have to do all of his chores, the door disappears. This also happens when Luigi is trying to listen in on what is happening inside the castle. (00:06:05 - 00:06:27)
Two Plumbers and a Baby - S1-E17
Revealing mistake: When Mario and Luigi look at what King Koopa is doing with the infants in the castle, one of the infants is laid out of the castle instead of inside it, and shortly after, a tricycle is laid over another tricycle. (00:06:31)