Visible crew/equipment: In the live-action segment, several times when Mario and Luigi confront Mrs. Gammliss, the shadow of either a crew member or a piece of equipment is moving at the top of the screen. It is not Mario, Luigi, or the Ratagator's, as they are not moving, and it is not the passersby's, as the shadow is present even when they are not present. (00:17:38 - 00:18:45)
Revealing mistake: In the live-action segment, when the glass in Luigi's hand shatters from Mrs. Gammliss' singing, Luigi can be seen actually pressing the glass together to make it break instead. (00:17:59)
Continuity mistake: After Luigi tells Mario to let go of the Bob-omb in the Mushroom Land, his black sideburns briefly turn skin colour. (00:15:51)
Revealing mistake: During Princess Toadstool and Toad's picnic, the tray of bread is layered over Princess Toadstool twice. (00:09:46)
Revealing mistake: When Mario heads to Luigi in one of the coaster cars, the car is initially to the side of the track instead of on it. (00:07:47)
Revealing mistake: After Luigi tells Mario to wait for him, he does not jump on one of the Koopa Troopas, but the Koopa Troopa reacts as though he did. (00:07:46)
Continuity mistake: When a Koopa Troopa trips a policeman, the policeman's baton disappears when it hits the ground. (00:04:48)
Continuity mistake: Before Mario and Luigi enter the taxi, Luigi's green eyes turn blue like Mario's. (00:05:08)
Revealing mistake: When a front view of King Koopa running down a street is shown, he clips through a building. (00:15:25)
Character mistake: When the Koopas chase Mario and Luigi, the sign says "COOPA ISLAND" instead of "KOOPA ISLAND." (00:06:39)
Character mistake: When Koopa is chased by a cop, he passes a Burger Shop. The sign says "BURCER SHOR" instead of "BURGER SHOP." (00:04:35)