Factual error: In this episode we have a random person showing up and being admitted to the team and playing as substitute on the spot, with nobody protesting. He puts on the (sweat-soaked, presumably!) uniform of another player and just goes ahead without any check from the referee or assistants. Given that the match is between two schools without necessarily having the official football federation involved, it is understandable, but they do not even ask the opposing coach if they are OK with something so obviously outside of any established rule. And they are still playing in a packed stadium, with official referees and even a radio commentator! Only in anime.
Other mistake: After his ankle injury from the previous episode, Ryo writhes in pain touching...his knee. (00:03:00)
Continuity mistake: When Wakabayashi dives and catches the cross as it's still high in the air, Misaki and Tsubasa are to his right, then to his left. (00:05:15)
Continuity mistake: Mr. "OONN", the goalkeeper of Urabe's team, gets to wear a uniform completely different from his teammate's, but we saw him walk into the stadium exactly with the same tracksuit as the others 2 episodes earlier. And that he's wearing again in this same episode after the decisive goal. (00:07:05 - 00:13:45)
Continuity mistake: Teppei Kisugi is blocked in his offensive spell by two Nankatsu players who change between shot and reverse; their hairstyles differ in the shot from the back compared to the two from the front. (00:07:20)
Continuity mistake: Taro Misaki's flashback starts with him juggling the ball as he wears a pink shirt and black pants. The pants change to green in the next shot, but even more surprising, he gets blue hair. (00:09:45)
Other mistake: Misaki's dad just arrived at the stadium, and seeing his son play, he strokes his beard with a satisfied look. Problem is, he's still standing and the hand that rubs his chin was holding a toolbox he did not have time to put down (or anywhere to place it on, for that matter). (00:10:40)
Continuity mistake: A shocked Wakabayashi is down on a knee after a second goal. The grey haired sweeper is the third from the right at first, but from Genzo's coach's POV he's the second from the right. (00:14:30)
Other mistake: There are just 9 players (plus himself) when Genzo rallies the team after the goal. (00:15:10)
Continuity mistake: The ball at the end of the game bounces on the post and stays on the field...except in the new shot it does not and goes out of bounds shaving the post. (00:18:40)
Continuity mistake: In the post-game handshake between Tsubasa and Genzo, the latter is wearing his gloves at the beginning, then, still with the hand in Tsubasa's, the hand is bare. (00:20:55)
Continuity mistake: Sanae is crying and wiping her tears when Tsubasa thanks her, she is happy smiling and clinging to the railing in the background when Roberto compliments Tsubasa, and again crying and wiping in the next shot. (00:21:45)
Continuity mistake: During Roberto's team talk, the keeper is wearing an ordinary jersey at first, then the proper red one. (00:01:50)
Continuity mistake: Ryo Ishizaki is wearing yellow boots at first, then blue boots when he's throwing balls to the training partners. (00:02:15)
Character mistake: At the tryouts, Tsubasa's teacher approaches coach Shiroyama chatting with surprise about the fact that he is in charge of the new team. But the episode opened with her exactly addressing him as the coach of that team. (00:04:40 - 00:19:45)
Continuity mistake: When the coach of the new Nankatsu team approaches the teacher, she picks the sheet with the players list and her left arm is not shown. In the wider angle the arm is right at the bottom of the sheet. (00:05:10)
Continuity mistake: When Roberto is looking at the team having a laugh with the still injured Ishizaki, there are 10 of them in the first shot with no keeper included and 10 including the keeper in the second. Both are wrong though, since Ryo has a substitute that is playing, so should be 11 players all along. Later in the episode when they run for the special training, it's 12 people, with Ishizaki still sidelined, but in the close-up of Roberto's foot you can see them run past, and it's 11 players. (00:07:05 - 00:10:10)
Other mistake: The Nankatsu teacher just announced the tryouts, and everyone responded positively asking for more training. Before Sanae starts her choir, the teacher is exactly the same height as the 10 year old students, contrary to the rest. (00:09:30)
Continuity mistake: When Genzo flips through the magazines, the cover is colored wrong in the first shot, with the cover photo filled in pink even if they went through the trouble of drawing it. In other shots it is properly colored. (00:11:20)
Continuity mistake: Coach Mikami's sunglasses are pitch black during most scenes, but in his reaction shot to the news of Roberto Hongo's international retirement they are tan. (00:11:35)