Chuck Versus the First Kill - S2-E20
Continuity mistake: There's a noticeable jump cut after Jill separates from Sarah - we see a cubicle, with a Fulcrum agent getting up and running off to the left, leaving his chair spinning slightly. He exits, then just before Jill enters (apparently in a continuous shot), the chair suddenly rotates to a different position and then stops.
Chuck Versus the Ring - S2-E22
Continuity mistake: In the wedding scene in the opening wide shot we see Morgan and the mic on stage before the actor goes on stage a good two minutes later.
Chuck Versus the Fake Name - S3-E8
Factual error: When they first bring Grueber into Castle, Sara states that "he is one of five people who can hit a target from half a mile away." Half a mile is 880 yards. Basically every person who has graduated from (or likely everyone who has been selected for) sniper school in any military in the world should be able to do this. (00:05:20)
Chuck Versus the Anniversary - S4-E1
Factual error: Chuck is supposedly riding a Los Angeles city bus. But the buses pictured are NYC buses. (00:14:05)
Chuck Versus the Suitcase - S4-E2
Factual error: During a fast serie of Milan and runways shots there is one pic of the harbour of Hamburg, Germany. (00:08:05)
Chuck Versus the Fear of Death - S4-E8
Continuity mistake: Near the end when Jeff and Lester confront "Greta" in the cage she pulls out her knife. Jeff attempts to disarm her but she cuts off his tie. She cuts it very close to the top, leaving very little around his neck, but when it cuts back to him after showing the piece hit the floor there is much more tie left.