Continuity mistake: During the car chase, the NSA's black SUV rams into Chuck's little car. The front license plate breaks off and is hanging on by one corner. When it's backing down the stairs, the plate has somehow reattached itself perfectly to the bumper. (00:29:20)
Continuity mistake: The size of the laser aimed at Chuck during the rooftop standoff changes in every shot. (00:33:35)
Continuity mistake: Sarah's stilettos transform into flats when running after Chuck through the pool. (00:34:50)
Deliberate mistake: Although it is later revealed that Sarah is the intruder, the person fighting is male.
Chuck Versus the Wookie - S1-E4
Continuity mistake: When the DEA agent Carina is running on the beach, she is wearing a white undergarment under her short black dress. Moments later she pulls off the dress and we see that she is now wearing a black bikini. (00:25:59 - 00:26:25)
Chuck Versus the Truth - S1-E8
Continuity mistake: Big Mike is talking to Morgan. When looking at the banana, it changes at the cuts in this scene.
Chuck Versus the Imported Hard Salami - S1-E9
Continuity mistake: In the scene where Sarah and Chuck find the suspected bomb, Sarah initially tries to disarm it by removing the numeric key pad under the display. She leaves the keypad off when she goes over to talk to Chuck. In the shots where the characters are talking, the keypad remains off but every time the shot changes to a close up of the suspected bomb the keypad is back on, under the display. (00:37:00)