Continuity mistake: After Buffy finds Dr. Gregory's glasses at the start of Miss French's class and places them on the table, they move position twice throughout the rest of the scene.
Plot hole: In "I Robot, You Jane", how exactly does Willow scan Moloch out of his book when the scan is only about two thirds the width of it? (00:04:25)
Welcome To The Hellmouth (1) - S1-E1
Continuity mistake: The scene where Xander is on his skateboard, he becomes distracted seeing Buffy walk up the stairs and accidentally hits the pole. When the camera pans back, you can see Buffy is out of view, and is nowhere to be seen. Because she was still at the top of the stairs when Xander hit the pole, you should have been able to see her walking in the background.
Never Kill A Boy On The First Date - S1-E5
Continuity mistake: When the vampire breaks the glass with his head, the remaining glass disappears.
Revealing mistake: When Buffy breaks into the locker, the locker is already damaged. Probably from a previous take.
Suggested correction: Watching the scene, the only real damage is a slight dent that isn't all that close to the lock (she basically punches out the lock to open it). And to be perfectly honest, a dented locker isn't out of the ordinary for a high school. I saw plenty of dented, bent and damaged locker doors all the time back in high school. It's entirely probable that it is damaged from a previous take, but I think it's also within that sort-of realm of possibility (since like I said, dented lockers aren't out of the ordinary) that it might not be considered a mistake. I'll leave it up to people's votes.
Welcome To The Hellmouth (1) - S1-E1
Continuity mistake: When Xander is talking to the girl at the Bronze his hand is on the pole and off the pole throughout.
Other mistake: The Master's skeleton, before it is destroyed, looks to be female and not male. (Note the wide hips and large birth canal-like hole.) Also in the Next season's "When She Was Bad," they have another shot of the same skeleton, however the hips are much smaller and more male. (00:43:55)
Nightmares - S1-E10
Revealing mistake: When Willow opens the door to the basement to look for Buffy, the shot looking up the basement's staircase shows Willow's shadow on the inside of the door. In the previous shot when she opened the door, it opens towards a wall and a staircase to the second floor. But there is no light source coming from the wall or the staircase, giving the impression that the basement was filmed on a separate set.
Audio problem: When Willow and Jenny jump in Cordy's car, Cordy mentions that she saw "these things coming at me!" However, her mouth isn't moving for the last few words.
Deliberate mistake: When Willow and Jenny go out to the parking lot only to discover vampires converging on the school, pay attention to the extras. You can make out some of the same extras in different shots coming from different directions. (Most notably, you can see a woman in a long black-and-white skirt coming from the direction of the parking lot, then a few shots later, she's suddenly over in the football field coming from there.) They obviously reused extras in different shots to "pad out" the crowds.
Continuity mistake: When Buffy descends into the Master's lair, the way she is holding her crossbow changes depending on the camera angle. Sometimes it's pointed straight forward, sometimes it's pointed upwards.
Continuity mistake: When Giles and Jenny are talking about Buffy, Giles says "We must get in touch with this Brother Luca chap!" watch Giles. In the first shot he's still reaching towards the top of the cabinet. Instantly in the next shot, he's in the middle pulling something down from the top of the cabinet.
Continuity mistake: When Giles and Jenny are talking in the library, when Jenny closes the bible, the way she's holding it changes instantly between shots.
Continuity mistake: When Buffy is consoling Willow after she discovered the dead students, as Buffy leaves the room, Willow's mouth is open and she looks down. It then cuts to a wideshot. Instantly, Willow's mouth is closed and she's staring forward, and she looks down a second time.
Continuity mistake: When Jenny meets Giles after Xander's botched attempt at asking out Buffy, the way she's holding the folders to her chest subtly changes repeatedly between the closeup and wide shots.
Continuity mistake: When Buffy turns down Xander's date proposal at the start of the episode, throughout the scene, Xander has his backpack up around his right shoulder. However, when he walks away, it's instantly down by his side.
Visible crew/equipment: When Buffy meets Giles in the library after the opening credits, and they start talking, throughout the scene, you can see set lights and reflectors reflected in Giles' glasses multiple times. This happens often throughout the series, but is particularly noticeable here.
Revealing mistake: When Willow says "The usual " at the beginning of the episode and it cuts to Buffy landing on the ground, you can briefly but see the stunt woman playing Buffy's face in the first shot for a solid second or two. That definitely isn't Sarah Michelle Gellar.
Continuity mistake: In the beginning, when Xander is practicing asking Buffy out with Willow, at once point Willow puts one of her snacks (candies, I think) into her mouth and starts chewing in a medium/wide profile shot of the two. It then cuts to a closeup of her and instantly she's no longer chewing, and immediately places a second candy in her mouth. She didn't have time to stop chewing and swallow between cuts.
Out Of Mind, Out Of Sight - S1-E11
Continuity mistake: When Cordy notices the word "Learn" in The Bronze and asks "What does that mean?" the positions of the ropes around her arms changes instantly between cuts.