Revealing mistake: In the scene where Ronnie visits Brundlefly for the second time we see Brundlefly walking on the ceiling and wall, however his shirt doesn't remain consistent with gravity. This is especially obvious when he pulls his shirt up to show Ronnie his new tumor and then places it back down. The shirt appears to be being pulled up to towards the sky from Ronnie's perspective. (01:07:30)

The Fly (1986)
1 revealing mistake
Directed by: David Cronenberg
Starring: Jeff Goldblum, Geena Davis, John Getz, Joy Boushel
Plot hole: The whole problem with the teleporter occurs when fly DNA is mixed in with Seth Brundle's DNA, starting his transformation into the Brundlefly. Brundle is on a hiding to nothing from the word go, and the fly is irrelevant. Humans are a walking talking mass of foreign DNA - we are host to one trillion bacteria all of which has a complete complement of DNA, as do various tiny mites that live in our hair follicles and all sorts of single cell organisms in our gut. If the transporter serves to mix the DNA of all living creatures which are in the transporter pod at the time Brundle would turn into a half-man, half-bacteria. Incidentally, DNA from a bacteria, an amoeba or a hair follicle mite would be just as 'compatible' with human DNA as that from an insect. It's quite a simple chemical, really.
Seth Brundle: I think you're making a mistake. I think you really want to talk to me.
Ronnie: Sorry, I have three other interviews to do before this party's over.
Seth Brundle: Yeah, but they're not working on something that'll change the world as we know it.
Ronnie: They say they are.
Seth Brundle: Yeah, but they're lying. I'm not.
Trivia: The film's director, David Cronenberg, has a cameo in this film as the doctor who helps Ronnie deliver her maggot baby.
Question: Well into the movie, Seth's computer, which uses voice recognition, can no longer recognize his voice due to his transformation. How then, did he hook up the three telepods and execute the final sequence if he couldn't get into his computer?
Chosen answer: He just used the keyboard. We see him typing on it several times before this scene, so the computer is not voice command only.
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Suggested correction: Being as how the bacteria and mites and such were IN or ON Seth, the machine was able to organize those symbiotic relationships accordingly as teleporting one would teleport all. The fly was separate, not touching. The machine was not programmed to anticipate two separated entities and so combined them into one on the other side.
Phixius ★
We leave behind a vapour trail of bacteria and viruses (among other things) as we walk, in our breath and emanations from pores in our skin, and Brundle isn't trapping any in his clothes as he isn't wearing any. Brundle has an invisible cloud of DNA floating around him in that teleportation chamber and as far as the machine is concerned their DNA has exactly the same status as that of the fly.