Corrected entry: When Chuck sees the Intersect images for the first time, it takes hours for him to see them all (as the scene stretches from the end of the party to the next morning when his alarm beeps for work). Throughout the series, many other characters upload the intersect and yet for them it never takes more than about a minute to see all the images.
Chuck Versus the Sizzling Shrimp - S1-E5
Corrected entry: A title card at the beginning of the episode identifies the location as the Chinese Consulate in Chinatown, Los Angeles. The Chinese Consulate is at 443 Shatto Place, west of Downtown Los Angeles and even farther west of Chinatown.
Correction: Addresses and buildings are often substituted, and is a standard filmmaking convention, not a mistake.
Corrected entry: In the first episode, we see Bryce Larkin "stealing" the intersect but failing when John Casey shoots him down. We later find out that an underground organization called Fulcrum has picked up his body so as to revive him and gain intel from him, etc. Later, after Bryce's "resurrection," when Bryce runs into Chuck and the crew, he explains that it was Fulcrum that wanted him to acquire the intersect for them. However, this isn't possible considering that they couldn't have sent him to gain the intersect because he didn't die until his attempt at it, and they didn't recover him until he died.
Correction: Prior to his attempt to "steal" the Intersect in the pilot, Bryce was contacted by Fulcrum. After he was contacted, he found out that they were trying to download & destroy the Intersect. So Bryce double-crossed Fulcrum by stealing it first and sending it to Chuck. Then Tommy (the agent we see in the flashback) revived him to find out what happened to the Intersect.
Correction: In the first episode Chuck is receiving the intersect as an email attachment on his computer. All other instances of an intersect being downloaded involve specialised devices, multiple screens, high-tech glasses or whole rooms or are updated forms of the intersect.