Continuity mistake: In season 1 Lincoln says his mother died of liver cancer. In season 4 they said she died from a brain tumour.
Character mistake: Mahone is very smart and meticulous, similar to Schofield. He has a good knowledge of forensics as shown throughout the show. However he shoots Tweener, and places the gun in his hands after shooting him. He would know that the blood on Tweeners hands would show he wasn't holding the gun when he was killed due to blood spatter behind behind the weapon. (00:40:20)
Continuity mistake: When Brad threatens his ex-cop friend Gary on the phone while leaving a message for him, he says "you better get down your knees and pray to God that I don't find you". Later in the court the recording is "you better get down your knees and pray to God that I never find you".
Continuity mistake: When John and Michael meet with Philly, he has his hands at his sides. Camera changes and they are on the table, clasped. (00:20:40)
Under & Out - S3-E11
Revealing mistake: When Link is dabbing Sofia's burns its clear to see he's not actually making contact with her. Just making the motion. (00:26:10)
Under & Out - S3-E11
Continuity mistake: When T-Bag and Lechero are in the tunnel talking about Michael planning something, Lechero has the crowbar level with his face in some shots and lowered in others. (00:14:00)
And Then There Were 7 - S1-E11
Factual error: D.B. Cooper made his escape on November 24th 1971 with 200k in $20 bills. Westmoreland shows Michael a $100 bill saying it's from the ransom. Impossible. (00:36:00)
Other mistake: When Sara's dad is found dead, Sarah pulled him, so his body dropped on the floor with his key, but this key is different from the other square key that they used it to open his private locker. (00:16:30)
Revealing mistake: When 5 of the escapees are heading to Oswego to get the supplies and Michael tells Lincoln to turn right, you can see it isn't a real car as the steering wheel doesn't turn - Lincoln just moves his hand along the wheel. Watch the grooves and you will see. (00:18:50)
Continuity mistake: Lechero regains command of the prison and dumps the bodies of the two guys off the ledge. His face goes from being clean to full of sand to clean in three short shots. (00:36:20)
Deliberate mistake: *SPOILER ALERT* Behind T-Bag is the actor who plays Lechero, and Lechero had just died in the episode. The person was not Lechero, but maybe they didn't have another extra at hand to replace him. 'Time 1' and 'Time 2' are taken from Netflix at the time this was posted. (00:29:46 - 00:30:25)
Continuity mistake: After Sucre and the driver of Stuart's car wreck, another car pulls up behind and starts honking. Cut to a shot from behind the cars and there is a whole line of cars waiting. They weren't there in the opposite angle.
Rendezvous - S2-E10
Plot hole: Linc is arrested in Wilcox, Arizona and is rescued by his father's people. They then take him to Trinidad in Colorado, an 8 and a half hour journey by car - they are seen in the same van. However in those 8 hours, it's not noticed that a) The police unit had been attacked and b) Lincoln Burrows, one of the 8 most wanted men in the USA, had escaped. Surely in 8 hours someone would have noticed. (00:07:35)
And Then There Were 7 - S1-E11
Visible crew/equipment: Cherry pickers and lights are visible in front of the main entrance. (00:31:59)
Continuity mistake: You can see David with his hands behind his head, but in the next shot he still has his hands beside him, and only later places them behind his head. (00:34:48)