Continuity mistake: When Princess Toadstool feels sorry for Toad, her blue brooch turns yellow, while the yellow border turns blue; and shortly after, it disappears. (00:04:59)
Continuity mistake: In the live action segment, when Mario and Luigi are fixing the pipe, Nicole is holding a newspaper, but when she is sprayed by the water, it disappears. (00:03:29)
Continuity mistake: After Mario drains the dungeon, Toadstool and Toad jump off the shackles they were hanging off. However, when Mario jumps off, Toad is again seen hanging from the shackles even though he had previously jumped down. The next scene confirms this by having him on the ground where he belongs. (00:10:28)
Continuity mistake: When the mother Birdo lands at her house, the chairs at the table have their backrests without cushions, but in the next shot, cushions appear on them. (00:03:45)
Continuity mistake: When first showing the Mushroom Land during the animated intro, there is no path present, but in the next shot, one appears. (00:03:47)
Continuity mistake: When Mario moves a curtain in the animated intro, there is no cavity in the shower, but in the next shot, one appears. (00:03:35)
Continuity mistake: When the magic carpet moves upward after Mario becomes Super Mario, Luigi's visor goes from green to skin colour and back to green.
Revealing mistake: When the Mario and Luigi in real life appear from the left side of the screen, the green or blue screen is not formatted properly, causing them to appear out of nowhere. (00:00:43)
Continuity mistake: When the limousine drives down the street, it has the US and USSR flags on the front of the limousine, but when water pushes it back, the flags change to generic red and yellow flags. (00:12:19)
Continuity mistake: When the crocodiles approach the general store, the store's front door has its doorknob on the left, but in the next shot, it appears on the right. (00:06:26)
Continuity mistake: When the ketchup lorry crosses the bridge, it has a cream cab and a red trailer, but in the next shot, it has a blue cab and a grey trailer.
Revealing mistake: When the camera pans across the front of Double Cross Ranch, the edge of an animation cel is visible. (00:13:51)
Revealing mistake: When a front view of King Koopa running down a street is shown, he clips through a building. (00:15:25)
The Koopas are Coming! The Koopas are Coming! - S1-E35
Continuity mistake: Just before the pillory is destroyed, it is violet, but in the next shot, it is orange. (00:14:37)
Audio problem: When King Koopa throws the snake at the Mario Bros., they use Luigi's voice when King Koopa says "Quick, quick! Run away from him!"
Continuity mistake: This mistake is in the intro with Mario and Luigi in real life. When Mario breaks the ceiling while Bowser is holding a yellow bar, Mario's gloves disappear from his hands when he jumps to break the ceiling. When he lands, his gloves reappear back on his hands. (00:00:26)
Revealing mistake: When the camera scrolls to Grunt (in the scene where the gang are locked up in a cell, being watched a strong, non-human security guard named Grunt), he appears out of thin air. The animators might have had some trouble putting him into the scrolling shot. (00:11:15)
Character mistake: When the Koopas chase Mario and Luigi, the sign says "COOPA ISLAND" instead of "KOOPA ISLAND." (00:06:39)
Character mistake: When Koopa is chased by a cop, he passes a Burger Shop. The sign says "BURCER SHOR" instead of "BURGER SHOP." (00:04:35)
Character mistake: The title card says "Plummers Academy" instead of "Plumbers Academy."