Continuity mistake: In the English dub, during the intro, when Team Rocket's Pokémon appear, the red in Arbok's pattern turns purple. (00:01:13)
Continuity mistake: In the Japanese version of the intro, the first shot showing Ash and friends shows Ash's bag with the label green. However, the label is white instead outside of this intro. Also, the front of Ash's hat goes from white to black and back to white. Additionally, part of his right glove goes from green to skin colour. (00:00:19)
Other mistake: In the English dub, when Bayleef jumps up in the opening, her left pupil is mistakenly coloured white instead of black. (00:00:47)
Other mistake: In the English dub, when Bayleef jumps up in the outro, her left pupil is mistakenly coloured white instead of black. (00:21:00)
Answer: Yes it ran away because Layla hurt its feelings.