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Continuity mistake: In the laser corridor, the team leader is stood upright when the lights comes on, but as the camera changes to a front view, he is crouched. (00:30:10)

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Other mistake: During the scene with the laser, the laser moves along the floor where the EMP device is located, several times. However, when Kaplan and Alice enter the room, the bag with the EMP unit is still intact. (00:31:15)

Legendary

Deliberate mistake: When the woman is attempting to escape from the elevator, it dislodges and falls down, so then the actress is facing the ground...but when her arm hits the floor, the floor itself bends. If you watch the DVD with the commentary on, the director points out that it was a break away floor in case the actress' head accidentally snapped forward, to avoid any injury. (00:08:00)

Revealing mistake: When the team leader is opening the door to the Red Queen chamber, he clips an electronic device to the door to hack the code. The device is actually a blood glucose monitor as used by diabetics - an Accu-Chek, I think. (00:31:05)

Trivia: All the cast and crew (including the producer) had to undergo a grueling one week of military training (which they dubbed their 'week of hell') under the supervision of a battle hardened ex-navy SEAL. By the end most people were naturally eager to give their trainer a little of his own medicine. Miss Jovovich (Alice) did: he's the zombie she head kicks into the test-tube cabinet and then steals the gun from (before fighting the zombie dogs). What sweet justice.

Revealing mistake: When Alice hits the first person in a biohazard suit, the padding is visible on the chest section. (01:25:40)

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Revealing mistake: When Alice hits the zombie Spence in the head, she misses Spence by a lot. (01:18:50)

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Continuity mistake: When Spence has been killed we see the broken monitor which has a white column through the screen, which turns black or white depending on the shot. (01:16:00)

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Continuity mistake: When Kaplan is walking through the corridor to get the EMP and head into the Red Queen's chamber, Medic's legs are together. When we see all the power shutting off, her position has changed and now her legs are apart. (00:32:40 - 00:35:50)

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Revealing mistake: When Kaplan is trying to turn off the lasers, take note of his keyboard. His fingers aren't touching the keys. (00:31:20)

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Continuity mistake: When the SWAT team arrives at the hive, the open an elevator shaft and one of them lights a flare with 2 hands. As the camera changes, he is now holding it at arm's length and with one hand without enough time to do so. (00:23:05)

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Continuity mistake: When we see the extreme close up of the robotic arm picking up the blue vial, the grip is uniform and parallel to the edge of the sliver end cap on the vial. When we see the vial a second later, the arm's grip has changed and is no longer parallel or uniform on the vial. (00:02:10)

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Other mistake: When we see the numbers for the coded entry to Alice's weapon, the order of the numbers is 1 - 2 - 3 - 4 - 5 - 6 - 7 - 8 - 0 - 9. The 9 and 0 are the wrong way round. (00:47:40)

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Continuity mistake: Alice's scar on her left shoulder changes shape and redness throughout the film. (00:09:05 - 01:25:00)

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Continuity mistake: When the Red Queen kills all the workers in the main office, the positions and actions of people differ from the video footage to the actual events. (00:06:35)

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Continuity mistake: When the first laser passes through it hits the one guy in the wrist, however we see him on the floor missing half of his fingers and hand instead. (00:31:30)

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Continuity mistake: When the Lasers have finished killing everyone, the EMP equipment is next to the glass wall. When Alice walks over and looks in to the corridor, it's moved. (00:32:20)

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Continuity mistake: When we see a CGI map of The Hive right before the Laser Room, the 3D thermal camera reveals a very rigidly stood up human body looking through the door's small window. When the camera cuts and we actually see Alice look through the window, she's slightly bent down so her head is on window level since she's taller. It's quite possible the CGI team just didn't spend more time than necessary to move the thermal human model's limbs and kept it in an almost default T-stance position. (00:29:30)

Continuity mistake: When Rain tries the door on the train and fails, we see her face and her hair has 2 strands on one side of her face. Camera changes and now she's got 1 strand. (00:16:50)

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Continuity mistake: The guy states that the room is sealed and his facial expression is one of panic. Camera cuts and now he's facing a different way and his expression has changed completely. (00:05:20)

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