Deliberate mistake: There was no possible way for Edith to still be alive after the spiked coffin closed in on her. There are spikes on the doors and at the very back and sides of it, and she couldn't have squeezed in to brace herself. Probably done for obvious reasons, but still a mistake nonetheless.
Factual error: When the moon is regrowing, it destroys the ship (which is pushing it further into the sky), yet the moon is still pushed into the sky anyway, even though there isn't anything to push it. Even if only the cockpit was destroyed and the rocket's engine was still there, it still wouldn't have been strong enough to push the moon into orbit anyway.
Continuity mistake: After Gru tells the first minion "Minions assemble", that minion slides down a pole and relays the instructions to other minions. After he does this you can see a minion standing on the right of a blue tube and he jumps in, after the camera begins to pan to the left, that same minion instantly appears back at the entrance of the tube. Probably an error in the animation sequence which returned the minion to his starting position when his animation was finished.
Character mistake: After Gru tucks the girls in for the night, Dr. Nefario tells him that there are 48 hours until the rocket's scheduled launch. The rocket launch, then, should have taken place at night, around the time the girls go to bed, two days later. The rocket, however, launches during daylight hours.
Continuity mistake: When Gru tells the minions what he's going to do, one of the minions take out a torpedo. The angle of the torpedo changes between shots.
Continuity mistake: When the girls are trapped in Vector's glass bubble, Margo's sweater changes from having rolled up sleeves to normal. Especially visible when the bubble smashes.
Continuity mistake: When Gru is explaining his plan to the minions, one of them takes out a torpedo and fires. The torpedo is held by one of the taller minions at first, but after the torpedo has fired and Gru says "Hey, Dave", we can see the torpedo was held by one of the smaller minions.
Suggested correction: The minion holding the torpedo was a small one all along. The taller one is probably just one in the crowd. If you look closely when the minions grab their weapons, Dave, the one who held it in the first place, is a short minion holding a torpedo.
Continuity mistake: During one of Gru's attempts to break into Vector's house, he is targeted with many bombs. In the first shot, only one of the bombs is colored red on the end. In the next shot, at least half of the bombs are colored red on the end.
Continuity mistake: The tourist van drives past an Egyptian man. Every other shot that shows the desert in the background shows no sign of the Egyptian man. Not even a sign of him walking back the way he came.
Continuity mistake: In the scene where Gru steals the shrink ray, his minion puts it in a glass thing. The minion doesn't close the glass door on it, but when it shows Gru and his other minion laughing for victory, the glass door is closed. And the minion didn't have enough time to close the glass container thing.
Continuity mistake: In the scene where Gru goes to get Agnes, Margo, and Edith from Vector from his "lair" (after giving him the moon) he puts his face against the camera and it displays Gru as taking up the full screen. When he then punches the camera it shows only a small portion of the screen is taken up.
Continuity mistake: When Margo is reading out Vector's cookie order to Vector, Edith and Agnes are seen putting the last six boxes of cookies on the table. The cookies aren't there in the next shot.
Continuity mistake: The tourists disappear in the shot where the fake pyramid is shown falling down.
Continuity mistake: Gru enters the Bank of Evil and starts walking through a corridor. As he starts to walk, he begins to walk past the first pillar. In the next shot, he has suddenly walked past at least 3 pillars.
Other mistake: While Gru is shrinking the moon the camera revolves quickly around the moon as it shrinks. When it cuts to the minions watching from earth, they have the same perspective as us.
Continuity mistake: When Gru takes the girls to his house just after adopting them, there is a newspaper on his lawn, lying completely on the grass. In the next shot, part of it is on the dirt pathway.
Continuity mistake: When Gru is hit by all of the missiles and guns that pop out of Vector's fortress he is standing right outside the large door, but in the next shot after he emerges from the crater he is in the middle of the street.
Continuity mistake: When Mr. Perkins is looking at a picture of him and Vector, he sets the picture frame down. In a few shots, the frame disappears.
Continuity mistake: Edith disintegrates Agnes's unicorn and a pile of ashes is left on the ground. In two shots, the pile of ashes moves slightly further from Agnes.
