Continuity mistake: After the Zombies are unleashed in the desert scene, we see Carlos crawling under the truck. At first his semi-automatic gun seems to be completely normal. The gun's slide and chamber are closed as if there are still bullets in the magazine or at least one bullet is chambered. But then the camera focuses on his gun and we see the gun's chamber is open - as if there are no bullets left. The gun didn't fire between shots so it's chamber should have been open in the first place. (00:58:10)
Continuity mistake: In the scene where Dr. Isaacs escapes from Alice in a AS350B Eurocopter, we see that the helicopter's landing gear has an additional iron bar that has been changed from one scene to another. When the helicopter lands in the desert, the bar is missing. So Dr. Isaacs flew away in one helicopter and landed in another. (01:04:00)
Continuity mistake: When the tanker explodes that Carlos jackknifes at the end scene, the zombies that surrounded the tanker simply fall over from the explosion. However when Alice drives the H2 up to and through the wreckage the ground is clear of any fallen bodies. (01:10:50)
Continuity mistake: When Issacs is choking Alice, she's seen to stamp and break the floorboards. Camera changes and the floor has been repaired and Alice's position has changed. (01:20:20)
Continuity mistake: When Claire approaches Alice who is watching Issacs fly away, Claire has her hands behind her back in one shot and up at her face in another. (01:05:00)
Continuity mistake: Alice steps into the entrance to the hut with her right foot in one shot and her left in the following shot. (01:12:50)
Continuity mistake: When Project Alice starts to fight the control of Umbrella she jumps off a wall. In the first frontal shot her coat is flowing freely behind her. But in the second shot, it's wrapped around her leg. (01:03:30)
Continuity mistake: When Chase falls off the Eifel tower, there is an overhead shot where it shows he's got a clear path to the sand. However he lands on part of the structure. (01:03:00)
Continuity mistake: Betty gets attacked by the birds and ends up with blood all over her face. When she shuts herself in, the blood is all gone and her face is clear. (00:39:00)
Continuity mistake: When Stevie is shot, there is blood spatter on the truck. When we see the close up of him the blood spatter has gone. (00:19:00)
Continuity mistake: Alice jumps up to avoid the laser at the very start and the laser cuts part of her dress off that is to fall to the floor. However when she lands the separate part is not on the floor where it should be. (00:03:30)
Continuity mistake: Carlos dives under his truck and finally shoots the two zombies in the head and his scarf is not around his mouth. As the camera changes, it suddenly is. (00:59:45)
Continuity mistake: Alice exits from the gas station and gets on her bike to read the book she obtained inside the gas station. However we see her looking at a page with a picture and the word "Isolated" on it. When the camera changes and she is closing the book it's on a different page. She's not changed the page as it's audible when she does and the sound is missing. (00:20:15)
Continuity mistake: When Stevie is against the truck, his head is tilted to his left while impaled. But in the close up of him being impaled his head is looking straight ahead. (00:18:30)
Continuity mistake: When Stevie is impaled on the abandoned truck, he's seen in a strip of shade, when Alice walks over the strip of shade has gone. (00:18:30)
Continuity mistake: At the start, the woman falls to the floor and you can see her kneepads under her trousers. (00:04:15)
Continuity mistake: At the start, the trolley gets cut in half and Alice isn't stood near either of the two halves. When she starts walking off she's next to part of the trolley. (00:04:10)
Continuity mistake: When Dr. Isaacs is injecting a zombie with his domestication serum, the head restraint on the zombie disappears. (00:26:10)
Continuity mistake: When Dr.Isaacs is injecting a zombie with his domestication serum, he changes positions from being beside the zombie, to being on the opposite side of the table. (00:26:10)
