Continuity mistake: When Carlos the bomber goes through the security door at Miami airport, he enters the door from a brightly lit area. We then cut to him emerging from the door - behind the door is completely dark. We then cut back to the door closing from the other side, showing that it's definitely intended to be the same door.
Visible crew/equipment: In the scene where Bond is following the bomber in Miami Airport, when the bomber goes through the security door using the ellipsis password, and proceeds down the corridor,Bond tries to catch the door before it shuts. There is a hand visible which pulls the door shut for a brief second and holds the door in place.
Visible crew/equipment: Lighting equipment is visible in Mathis' sunglasses, just before he is shocked while talking to Bond.
Factual error: The name of the bank holding the winnings for the poker game is incorrrectly configured. The formulation of banks referencing cities in Switzerland (and Germany) are such that the name of the "Basel Bank" would actually be the "Basler Bank" in the same way that the "Zuerich Bank" would be "Zuercher Bank," usw. It is not a mistake that there is no such bank as the Basel Bank (because some bank would have to pay for such excellent product placement). However, there would never be a bank named Basel Bank in Switzerland. It would be named Basler Bank.
Continuity mistake: During the 1st break of the big game Bond is all covered with blood and his hands and face are wounded. After he washes the blood and changes his shirt he returns to the game and his wounds are healed.
Revealing mistake: When Bond is running up the crane to catch up with the bomb maker at the construction site there is traction tape, to help Daniel Craig keep his footing on the smooth metal surface, visible on the crane. It wasn't put there as though for the construction crew's use because it is only placed on the part of the crane that we actually see Bond running on.
Continuity mistake: After the torture scene, just after La Chiffre says he will feed Bond his "parts" you hear a scream from within the ship and then a gunshot. The camera shot then focuses on the ship's hatch. The handle on this door has changed from when the rest of the group went into the ship. The handle is now pointed to the right in the open position instead of straight up as it was when they locked the door. Just before "Mr. White" comes out of the hatch, you can hear the sound of the handle moving to the open position without any movement of the handle.
Continuity mistake: When Bond is following Vesper, when he turns right to follow her through the alleyway the street behind him gets a lot more populated between shots.
Revealing mistake: During the close up shots of Le Chiffre when he's torturing Bond, the contact lens used to make his left eye pale blue is visible.
Factual error: In one scene, Bond blows up a propane tank with his 9mm. But, the 9mm couldn't possibly puncture a tank like that. And, even if it could, the result wouldn't be explosive. It would let off a lot of gas, but that's it. You would need incendiary rounds to do anything remotely similar to what we see in the movie and a much bigger, higher velocity round. Mythbusters tested this scene and even an armor piercing 9mm round couldn't penetrate the tank, nor could incendiary rounds ignite it.
Continuity mistake: In the poker scene where Le Chiffre beats Bond with having four Jacks vs Bond's full house of Aces and Kings, we first see Bond having the Ace and King of spades, but when he reveals his cards they have changed. We now see Bond with the Ace and King of hearts.
Audio problem: In the scene in the shower when Vesper says that she feels she has blood on her hands that won't come off, her mouth says "hold me" but she actually says something different. Also she says "please" but nothing is heard.
Continuity mistake: In the second half of the scene with Bond and Vesper seated across from each other on the train, Vesper's armrests are down in the shots showing both her and Bond but they are up in the close-ups of her face.
Factual error: When Bond retrieves the bombmakers's phone in Madagascar, he finds the Ellipsis message with a time code of 19:12. After tracing the origin of the message to the Ocean Club in the Bahamas, he finds the security footage of Demitrios sending the message at 19:12. But 19:12 is the local time for the Bahamas, as indicated on the surveillance tape and Demitrios' phone. Madagascar is 8 hours ahead, so the bombmaker's phone should have indicated the message was received at 3:12 a.m. Also, there is a lot of sunlight and activity going on for 3 in the morning.
Continuity mistake: When the house in Venice is collapsing - and it is shown from the outside - it moves quite fast. When it is shown from the inside it is not moving at all. Also the inside scenes last so long that based on the speed seen on the outside, the house would have been submerged long before the fight ends.
Other mistake: During the chase with the fuel truck at the Miami airport, Bond depresses the brakes, which apparently aren't working, and the truck rolls on. Two problems: first, after almost hitting the plane and sliding sideways, the brakes skid to a halt and you can hear air discharging from the brake system. Which brings up the second problem: since the truck is air brake equipped, there is no way it would have continued rolling if the brakes were malfunctioning. Part of the air brake system on all trucks since 1974, have a safety feature which "dynamite" the brakes if there is no air pressure in the system. This locks up the brakes with the spring brake system, making the truck stop. The truck was newer than 1974, meaning it would have had the CVSS safety braking system from the manufacturer, and would have skidded to a stop if there was no air in the system.
Continuity mistake: Near the end when Bond is following Vesper, the camera shows her from behind and then from the front, the people around her are completely different people.
Revealing mistake: Towards the beginning of the film, when Bond jumps off the van into the embassy, the patch of ground he lands on is obviously a landing mat with leaves and turf covering it.
Continuity mistake: Le Chiffre's scar on his eye changes colour, size and position during the movie. Most noticeably it is much more red and sharply drawn during the final poker game than it is in the opening scene in Uganda.
Continuity mistake: Near the start, when the Ugandan is thinking about the money, his hands are together in front of him. At first his fingertips are crossed, then in the next shot they're suddenly placed together.
Answer: From the novel: "Three measures of Gordon's, one of vodka, half a measure of Kina Lillet. Shake it very well until it's ice-cold, then add a large thin slice of lemon peel."
Captain Defenestrator