Audio problem: When the chief of police arrives at the blood farm he is surprised by Blade and Abigail. He quickly reaches inside his jacket to draw his pistol but Abigail draws her revolver much faster. As she draws there is the unmistakable metallic sound of a revolver being cocked, but when she extends the revolver out in front of her to cover the chief the hammer is down, not cocked. The revolver could be seen in the holster with the hammer down, so she could not have been manually decocking it to make that sound.

Blade: Trinity (2004)
1 audio problem
Directed by: David S. Goyer
Starring: Ryan Reynolds, Wesley Snipes, Jessica Biel, Kris Kristofferson, Parker Posey

Continuity mistake: When Blade has been rescued by Abigail and Hannibal and the 3 of them are approaching the hideout, notice in Abigail's back quiver there is one arrow left, yet in the next shot when they're walking up the stairs she has two.
Hannibal King: Welcome to the honeycomb hideout.
Blade: How do you bank roll this operation?
Hannibal King: I date a lot of older men.
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Answer: It's possible that he followed one of them back to the base. This is feasible since they wouldn't be able to tell that he was following them because of his shape-shifting abilities.