Continuity mistake: The Mario Bros. appear briefly without their tool belts after they enter the alternate dimension. (00:23:05)
Continuity mistake: In the devo chamber, when Toad is devolved the Advance sign is black. When they evolve Spike the sign is red. (00:36:20 - 00:46:45)
Continuity mistake: When Mario and Luigi are in the elevator with the Goombas they're still wearing their specialised jump boots. The instant they get out of the elevator they're back in their normal shoes. (01:06:30 - 01:10:50)
Continuity mistake: In Devo 4 Luigi's button unbuttons and rebuttons itself. (01:18:05)
Continuity mistake: When Mario and Luigi follow Koopa's goons into the cave, Luigi jumps through the portal in the wall but Mario is more hesitant. When he falls toward it, in one shot his feet have barely left the ground, but in the next shot immediately afterwards he is pretty much upside down as he falls through the wall.
Continuity mistake: A Bob-Omb explodes under the devolving Koopa and sends him flying. The shot shows him flying straight up, and without cutting, shows him begin to fall along the same trajectory. The big pot thing is off to his left, and well out of reach of him falling. Yet then it cuts to him falling at an obvious angle and into the pot. The trajectory was all wrong.
Continuity mistake: Just after the car chase scene, Mario drives the police car into a tunnel, which disconnects the vehicle from the power grid. The car even says, power shutting down, causing the monitor to shut down, and for the breaks to stop working. However, even after the car leaves the tunnel and is dangling in fungus, way out of reach of the power, the headlights and the police lights of the car are still lit. They should have shut down also when power was lost.
Continuity mistake: When Koopa is chewing out Iggy and Spike for forgetting about the rock, the amount of wax on his hands changes multiple times between cuts. For instance, the wax covering his left hand disappears in one wide shot, but is present in every other shot. Additionally, the amount of wax on his right hand changes in amount several times.
Continuity mistake: When Koopa tells Daisy that women "never forget the first time they get kissed by a lizard," his face starts to morph. It then cuts to the opposite angle, and suddenly, between cuts, his hand has appeared up by his face.
Continuity mistake: During the car chase, after Mario says "This is driving!" Luigi goes from facing forwards to facing sideways at Mario between cuts.
Continuity mistake: After Toad is turned into a Goomba, when Koopa is talking about his own evolution, Toad goes from being shackled to the chair to be unshackled and leaning forward instantly between cuts.
Continuity mistake: When Koopa is explaining the devo chamber to Mario and Luigi, we see a monitor showing an x-ray of Toad's de-evolution. When the camera cuts from the wide shot to the closeup, the monitor has suddenly gone back in the animation and replays the same segment again.
Continuity mistake: When Mario and Luigi are giving their names to the officer, at one point, another officer breaks a glass and we see shards of it landing on the book in front of the officer. A few shots later, most of it has vanished.
Continuity mistake: When Mario and Luigi are giving their names to the officer, there's a woman randomly rubbing her foot against his arm and shoulder throughout the scene. However, the way her foot is on him changes multiple times throughout the scene.
Continuity mistake: When Big Bertha picks up the old lady, she goes from holding her in front of her to holding her above her head instantly between cuts.
Continuity mistake: When Mario and Luigi are in the cave, Mario goes from Luigi's side to behind, to being on the other side between cuts. There's no point in time where either they (or their feet) aren't on camera, so it makes no sense.
Continuity mistake: When Daisy and Luigi are looking at the fossils, after Luigi says "You're beautiful," the distance between him and Daisy increases instantly as it cuts to a wide shot.
Continuity mistake: When Daisy is showing Luigi the fossils, as the camera cuts from the wide shot to the closeup (when Daisy says "Have a look!"), suddenly their heads go from facing up to facing down between cuts, as do their flashlights.
Continuity mistake: When Iggy and Spike round the corner and snatch Mario's girlfriend, the look on Iggy's face changes as it cuts from a close-up to a wide shot.
Continuity mistake: When Mario is watching Luigi trying to talk to Daisy after she gets off the phone, he is tapping the empty water bottle against his head. In the first shot, it's against his forehead, but in the next shot, it's suddenly down by his cheek.