Continuity mistake: Oliver chops off Malcolm's hand to end their duel. When Oliver hands the ring over to Nyssa you can see Malcolm lying in the background where he has been cut down with two hands intact, and the cut off hand nowhere in sight.
Visible crew/equipment: While the camera is moving from Black Canary and Speedy to Spartan you can see on the ground the shadow of the camera man walking. (00:33:45)
Factual error: Felicity tracks the abducted team members to latitude and longitude values that are negative, which she and others present regard as impossible results until they realise the captives are in outer space. In actuality, south latitudes are conventionally represented as negative, as are west longitudes; and negative values would not convey information about an outer space location, which would instead be referenced with celestial coordinates. (00:36:50)
Factual error: When Oliver is hunting Prometheus, they come face to face. Oliver fires an arrow, which is tracked in slow motion before being split by one of Prometheus' throwing stars. Problem is that Oliver's arrow is, well, flying straight as an arrow. But in reality, when you film an arrow in slow motion, they flex and wobble, and definitely wouldn't fly like the one shown on screen.
Crisis on Earth-X, Part 2 - S6-E8
Deliberate mistake: During the big battle, Arrow is knocked down and when he opens his eyes, the reflection in his eyes is tilted over to give the sense he's seeing things sideways. But the reflection should still have shown the proper orientation no matter what orientation his eye was at. Think of looking into a mirror and then rotating the mirror to the side, the reflection wouldn't rotate.
Factual error: The highest-ranking military officer in the DIA (Defense Intelligence Agency) is the director, which is a three-star position. John's stepfather Roy Stewart would not be a part of the organization, let alone answering to superiors in the organization if he was a four-star general.
