Continuity mistake: In the intro, the emblem on Luigi's cap is green, but throughout most of the episodes, it is black.
Continuity mistake: During the intro, Princess Toadstool's shoes are green, but in the episodes, they are pink.
Continuity mistake: When Mario and Luigi notice Yoshi eating the barbecue, the "M" on Mario's cap has its colours reversed, being white on a red background instead of the other way around. In the same shot, Luigi's legs are missing. (00:02:54)
Revealing mistake: When Luigi says that Yoshi can go to Mama Fireplant to get some fireballs for the barbecue, his mouth detaches from his face for a few frames. (00:03:09)
Revealing mistake: When Princess Toadstool says that Yoshi can take the bridge, she passes through a table. In the same shot, Yoshi overlaps on the table. (00:03:21)
Continuity mistake: When Kootie Pie picks up the shell, its green underside turns brown like its rim. (00:03:52)
Continuity mistake: In a couple of shots, when Kootie Pie and the Koopa Troopa are moving Mama Fireplant, Mama Fireplant's white lips turn brown. (00:04:08)
Other mistake: When Yoshi is holding onto the bridge, part of its sides turns transparent. (00:04:42)
Other mistake: When the Koopa Troopa slides the manhole back into place, part of it turns transparent. (00:04:55)
Continuity mistake: When Luigi is about to slip on the ice, the symbol on his cap has its colours reversed, with the "L" being white and the background being black instead of the other way around. (00:05:12)
Continuity mistake: When Luigi crashes into his friends, the white stripes on his and Mario's gloves turn blue, like their shirts. (00:05:18)
Continuity mistake: When Luigi crashes into his friends, Mario's white gloves turn the same colour as his skin, while the stripes turn blue like his shirt. This also happens when Mario asks where the ice came from. (00:05:21)
Other mistake: When Luigi mentions the last time he saw ice, he has a green "M" on his cap instead of a black "L". (00:05:36)
Other mistake: When Princess Toadstool says that she and her friends have to save Mama Fireplant, her nose briefly disappears. (00:05:45)
Continuity mistake: When Mario throws Luigi into the Warp Pipe, the ice under the grate disappears, making it appear as if the grate is resting on nothing. (00:06:03)
Audio problem: When Princess Toadstool jumps into the Warp Pipe, she speaks, but her mouth does not move. (00:06:11)
Continuity mistake: When Mario and his friends are about to land on the second platform, the spikes on Yoshi's back are missing. (00:06:36)
Continuity mistake: When Princess Toadstool catches Mario, her blue eyes turn black. This also happens when Mario jumps into the slush to save Luigi. (00:07:24 - 00:07:54)
Continuity mistake: When Mario and his friends are falling into Ice Land, the hand Mario holds onto Princess Toadstool with switches a couple times. (00:07:35)
Continuity mistake: When Kootie Pie steps on a porcupinefish, her yellow bracelet turns brown like her body. (00:10:29)