Continuity mistake: When Dr. Miranda Bailey tries to resuscitate/reanimate her patient Jeremiah, the assistant puts the anesthesia bag down next to Jeremiah's shoulder, in the next shot however, she has the bag back in her hands and lies it down once more.
Continuity mistake: When Alex walks over to the desk that Derek is sitting behind, with the couple arguing in the background, there is a box of tissues with a tissue pulled so that it is almost out. In the next shot the tissue is barely out of the box. (00:26:20)
Audio problem: When Alex is walking over to Derek, watch the couple (the man with the gunshot wound in his head and his wife) in the background. The wife is shouting at his husband but her lips are not even moving correctly. (00:26:20)
Continuity mistake: In the bar, when Bailey tells Derek to go sit by someone who cares, she lifts her glass up from the bar table, to her lips, twice between shots. (00:01:50)