Continuity mistake: After King Koopa says that he should take over Sky Land as well, his yellow eyes turn green. (00:08:43)
Other mistake: King Koopa is able to stick himself out of one of the windows on the Doomship, despite it being closed. (00:08:52)
Revealing mistake: When the hidden chamber is revealed, the background is that of the chamber instead of the basement. (00:07:44)
Audio problem: When the Mario Bros. are breaking blocks, they initially are not breaking any, but the sound of the blocks breaking can still be heard. (00:04:01)
Revealing mistake: When Princess Toadstool and Toad are running to Princess Toadstool's castle, they briefly appear frozen. (00:06:33)
Continuity mistake: When King Koopa says the Mario Bros. are in time for his plan, Luigi's green cap turns red, his black hair turns brown, and his green eyes turn blue, looking like Mario. (00:05:16)
Continuity mistake: When the Mario Bros. fly through a window, it is open, but in the next shot, it is closed. (00:05:08)
Continuity mistake: When Kooky says he upgraded his machine, the green rings around the spikes on King Koopa's shell turn orange, like the spikes themselves. (00:04:57)
Continuity mistake: When the Mario Bros. fly, Emperor Ed back up to Sky Land, his medals change from yellow, red, and white to completely yellow. (00:04:32)
Revealing mistake: When everything is returned back to normal, both times, a bowl appears from thin air, while a Mushroomer by a clothesline freezes. (00:03:17 - 00:11:08)
Other mistake: When Emperor Ed falls out of the Doomship, he falls through a closed window. (00:02:49)
Continuity mistake: When Emperor Ed falls out of the Doomship, his red sash turns blue like his shirt. This also happens when he revolts. (00:02:48 - 00:08:54)
Continuity mistake: When King Koopa says Kooky's device works perfectly, Kooky's green eyelids turn blue like his hair. (00:01:22)
