Continuity mistake: When Ash, Misty, and Brock turn to Woodruff after he says for Ash to wait, Ash's right glove goes from covering his thumb to not covering it. (00:02:23)
Continuity mistake: When Ash turns after he wonders how the Heracross will help the Butterfree, the marks on his cheeks go from being present to being absent and then back to present. (00:03:02)
Answer: Yes it ran away because Layla hurt its feelings.