Continuity mistake: When Tracey asks if Misty is coming with him and Ash, part of Misty's arms changes from skin-colour to yellow. (00:01:49)
Continuity mistake: When Misty steps on Tracey, Tracey's blue shoes turn black. (00:02:13)
Character mistake: Misty is notoriously afraid of bugs. She makes this clear whenever bugs are around. She seems to be OK with Tracey's Venonat, but for some reason doesn't think it should be classified as a bug, when its type is Bug/Poison, and it's quite obvious that "Venonat" comes from "venom gnat, " a gnat being an insect. She even directly states that "At least they're [Venonat and Marill] not Bug types." [This error doesn't happen in the Japanese version.]. (00:03:19)
Continuity mistake: When Ash throws a Poké Ball to catch Scyther, his bag disappears. (00:04:40)
Continuity mistake: When Lickitung runs into Victreebel, its pink eyebrows turn yellow. (00:17:15)
Continuity mistake: When Tracey recalls Scyther, the ring around the button on the Poké Ball disappears. (00:20:36)
Answer: Yes it ran away because Layla hurt its feelings.