Challenge of the Samurai - S1-E4
Continuity mistake: When Ash goes to get a Poké Ball for the first time, the ball is upside down, but in the next shot, it is upright. Also, behind the same Poké Ball, there is another one, but in the next shot, there is not. (00:03:19)
Answer: Yes it ran away because Layla hurt its feelings.