Continuity mistake: Fluttershy's neck disappears after she says, "I don't know if I can cheer enough to help her".
The Elements of Harmony (2) - S1-E2
Other mistake: At the end of the episode when Princess Celestia is talking to Twilight Sparkle you can see that Princess Celestia's tail is on top of her leg instead of in-between. It's covering her cutie mark.
Continuity mistake: When Rarity levitates the stick to mark a spot where she detected the gems, she drops the stick right after a guard "threatens" her. Then, in the same scene, the stick has mysteriously disappeared.
Trivia: There are a lot of Greek references. Cerberus, Tartarus, a messengers boots (Hermes reference) and the princesses. Twilight is the Greek word for friend love, or Philia. Cadence is Eros, or romantic love. Celestia is Agape, the love God has for mankind. Well, Celestia is kind of a god. Luna is Storge, love of a mother and child. This works because she has helped Scootaloo and Sweetie Belle in a motherly way. She was a mentor to both of them.
Trivia: Pinkie Pie didn't use the trombone to get rid of the parasites. That means she planned for the exact ending. Not unlikely, considering Pinky Sense.
Discord: 'What fun is there in making sense?"
Rainbow Dash: Oh you want to do this the hard way? we do this the hard way!
The Elements of Harmony (2) - S1-E2
Question: When Rainbow Dash and Fluttershy are floating with Twilight, Nightmare Moon, (In the form of smoke) floats right in Twilight's face, though she never notices it. Why is that?
Question: During the song, 2 cute bats are seen with 'Jude'. When the cute bat couple is shown again, Jude is nowhere to be seen. The couple doesn't look at all concerned. When Applejack was singing "These creatures have a one-track mind!", Fluttershy appeared without Jude and couldn't take him or her home and then come back in about 5 seconds. So, where did Jude go?
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Answer: The smoke floats a few feet away from Twilight and close to the camera so you might think that the smoke is right in her face.
Lily Harrison