Other mistake: In the cupcake shop, Lucy crushes several cupcakes. These cupcakes have icing on them. But no icing goes on Lucy's hands at all. (00:24:35)
Other mistake: As Eduardo goes to leave the cupcake shop, Gru believes he could be El Macho and Eduardo leaves. Gru tells Lucy about El Macho for about 90 seconds, then Eduardo is seen from outside the cake shop window only just about to go up the escalator, when the distance between the shop and escalator is only a few meters. (00:27:55 - 00:29:25)
Other mistake: When Agnes screams after the evil minion tries to eat her unicorn, the noise shatters glass items and a sculpture on the mantelpiece as well as the evil minion's goggles. But Margo's glasses do not appear shattered at all.
Suggested correction: Glass shatters due to a loud sound because of a phenomenon called sonic resonance. If the glasses' natural vibration frequency doesn't match the frequency of the scream, they won't shatter.
Suggested correction: Her lenses could very well be made of plastic, which would not shatter like glass.
Other mistake: After Gru has left El Macho's lair, Gru goes to get the girls and finds Margo sitting at a table eating from a nacho hat that has guacamole in it. The hat is slanted sideways, but the guacamole remains in fixed position and never slides to the side. (01:09:20)