Continuity mistake: After S.H.I.E.L.D "borrows" Jane's research and Darcy says that they took her iPod, her hair can be seen blowing in the wind in the shots facing her left and not blowing in the shots facing her right.
Continuity mistake: When the Destroyer arrives amidst the Shield agents, a coffee cup sitting on the dash of one of the vehicles spills over. The next shot it can be seen lying on its side. The reverse angle shot then shows it upright again. (01:21:20)
Continuity mistake: At the end of the scene where everyone is trying to pull Mjolnir out of the crater, a SHIELD agent is seen pulling up in an SUV at the top of a hill about 50 yards away. When the shot changes to the view from where the SHIELD agent gets out of his SUV and is standing at the top of the hill he is a 1/2 mile away or more.
Continuity mistake: When Thor and friends first arrive on Frost Giants realm, rocks tumble and create debris - which disappears between shots.
Continuity mistake: When Thor is walking towards the Destroyer towards the end of the movie, the shadows of the Destroyer, buildings and cars change between shots. Also the Destroyer's height changes from being about 12 feet tall to 8 feet in those shots.
Continuity mistake: When Jane and Thor are driving out to where the hammer is, we see a lot of side shots showing barbed-wire fence posts passing by on both sides of the truck, but when Jane is distracted and goes a little off the road, we are shown a front shot of her bringing the truck back onto the road, except there are no fence posts or fence anywhere to be seen. Immediately afterwards, we again see side shots with fence posts going by. (00:51:00)
Continuity mistake: The first time Jane, Darcy and Erik meet Thor, Jane says "Do me a favour and don't be dead. Please. Where did he come from?" But the second time we see them meet him, Darcy suddenly says "Woah, does he need CPR? Because I totally know CPR" right after Jane says "Do me a favour and don't be dead". Extended scene or not, Darcy's line was not there before and it makes no sense for it to be there now because Jane's line is still incomplete when she says it. On top of this, the word "Please" that Jane said earlier is completely gone.
Continuity mistake: In the desert scene where Jane, Darcy and Eric are getting out of the van to check on the person they just hit, they rush to Thor who is lying on the ground and inside the "bifrost footprint" circle. There are 3 shots following the first wide shot, which focus on Jane, Darcy and Eric, and then back to the wide shot of the 3 surrounding Thor again, but the bifrost print is gone this time. After the Asgard backstory we return to the desert scene and the bifrost print is once again present on the ground.
Continuity mistake: When Thor is trying to recover his hammer from SHIELD, he rolls around on the ground quite a bit and is completely covered in mud. As he is being taken away by the agents at the end of the scene, though, his shirt is clean.
Continuity mistake: At the end when Loki goes to lock the bifrost on Jotunheim, he's wearing his helmet and carrying Odin's staff. He slides Heimdall's sword into the machinery to open the bifrost and his helmet is gone. He freezes the electrical streams then when Thor comes in to stop him, Loki is wearing his helmet again.
Continuity mistake: When Dr. Zelvig and Thor are drinking at the bar they get a mug of beer. The bottles of beer in front of them disappear between shots.
Continuity mistake: When Thor tries to get back his hammer, he makes a hole in the fence. Beside that point there are 3 barrels which disappear between shots.
Continuity mistake: When Thor dumps the table with food on it, the bread and cheese change position between shots.
Continuity mistake: After Thor gets the hammer back and he talks with Coulson and Jane, the sun changes between shots.
Continuity mistake: The nurse that holds Thor in the clinic has gloves but in the next shots the nurse is a different guy and the gloves are gone.
Suggested correction: The original nurse gets out of the way for an orderly.
Continuity mistake: Before Thor gets the throne of Asgard, there are 2 ice giants looking for the energy cube, but in the next shot there are 3 of them.