Plot hole: The snakes are contained in the cargo hold which is shown to be unpressurised on this aircraft (there is an altimeter which releases the snakes after it hits 35000 feet - if the hold was pressurised it would level off at cabin pressure, which is about 8,000 feet). A cargo door is left open to allow the snakes into the passenger compartment, but this would either cause the plane to not pressurise properly, causing everyone on board severe breathing problems if not worse, or would keep the hold at cabin pressure, meaning the altimeter would never reach the right level to release the snakes.
Snakes on a Plane (2006)
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Directed by: David R. Ellis
Starring: Samuel L. Jackson, Julianna Margulies, Nathan Phillips, Rachel Blanchard
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Neville Flynn: Enough is enough! I have had it with these motherfucking snakes on this motherfucking plane!
Trivia: Take a look at the button the flight attendant presses when he puts the snake in the microwave. It's conveniently labelled "Snake".
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Answer: It depends on whose airspace you are in when it occurs, whether or not anyone else sees you (public indecency), the laws of the nation that the airline is based in, etc.
Grumpy Scot