Plot hole: The snakes are contained in the cargo hold which is shown to be unpressurised on this aircraft (there is an altimeter which releases the snakes after it hits 35000 feet - if the hold was pressurised it would level off at cabin pressure, which is about 8,000 feet). A cargo door is left open to allow the snakes into the passenger compartment, but this would either cause the plane to not pressurise properly, causing everyone on board severe breathing problems if not worse, or would keep the hold at cabin pressure, meaning the altimeter would never reach the right level to release the snakes.

Snakes on a Plane (2006)
Plot summary
Directed by: David R. Ellis
Starring: Samuel L. Jackson, Julianna Margulies, Nathan Phillips, Rachel Blanchard
A man witnesses a gangster (Eddie Kim) kill a prosecutor who had tried several times to send Eddie to jail. The witness is then convinced to testify against Eddie. The murder occurred in Hawaii, and they have to fly to LA. Snakes are in the cargo hold on a timed cage (apparently security didn't catch them because they are cold blooded) and were, of course, placed there by Eddie Kim so that the witness would not survive. The first people to die are a few kids joining the mile high club and one of the pilots. Then snakes start coming from everywhere and the passengers have to fight them off/barricade themselves from the overly aggressive snakes. The second pilot ends up dead and a man with experience flying planes on his PlayStation2 ends up landing the plane safely.
Tenqit
Neville Flynn: Enough is enough! I have had it with these motherfucking snakes on this motherfucking plane!
Trivia: During filming, Samuel L. Jackson did not come into contact with any live snakes, because a contract clause prevented snakes from being within 25 feet (8 m) of the actor.
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Answer: Rattlesnakes only make a noise when they are disturbed and some rattlesnakes will even strike without starting to rattle first.
Myridon