Continuity mistake: Near the end of the film when we see Peter looking out of his window from his apartment, the girl from across the hall offers chocolate cake. When they are done eating, Peter is holding the fork with his thumb and in the following shot he is not. The fork has moved across the plate a little between shots. (01:17:30)
Continuity mistake: There are two close-ups of Peter's head when he is lying on the floor of the train: one of the shots has a black shoe with white laces very near his head, the other a blue, white, and black shoe, also right next to his head. In the wider overhead shots of Peter, no one standing around him is wearing shoes like that, nor are they standing close enough for their feet to be in the close-up shot in the first place.
Continuity mistake: When the garbage man is in Jameson's office, and Jameson is holding the Spider-Man suit, he is holding it almost at arms length to his right, yet in the following shot, it's a lot closer to his body. (01:05:50)
Continuity mistake: When the garbage man enters Jameson's office, Jameson is holding a phone to his chest, yet in the following shot he is not holding the phone, and we didn't see him put it down in the previous shot. (01:05:40)
Continuity mistake: When the people are watching Doc Ock with his experiment, Rosie is standing at the bottom of the stairs. When Doc Ock passes her his coat, she is then seen in the background standing next to a large TV screen. (00:32:35)
Continuity mistake: When Peter starts to lose his powers, and tries to climb up the side of the building, in several shots, he is by a window, and he slips opposite the window, yet in the following shot he is a lot further above the window. His distance and position from the window changes in subsequent shots. (00:56:30)
Continuity mistake: When we see MJ and her fiancee in her room close to the end, MJ says to him "Lean your head back for me." Her hand is not in his hand in one shot, but is in another. (01:23:40)
Continuity mistake: When Doc Ock escapes from the hospital, he hits a taxi with his tentacle. The taxi goes flying and is about to land on its roof in the middle of the street. The next shot however shows the taxi landing on top of another one, and the taxi is right side up.
Continuity mistake: After Doc Ock throws the car through the side of the deli, it shows Peter and MJ from outside the building and there's a table with a chair at the base of the hole right up next to the nonexistent wall. For the rest of the scene, the table and chair are not there.
Continuity mistake: When Doc Ock robs the bank, Peter pushes Aunt May away, but in one shot he turns his chair to push away, yet in the following shot the chair is facing a different direction. (00:46:10)
Continuity mistake: When Spider-Man is on the train with Doc Ock, Spider-Man saves two people and in this shot we see a station, yet in the following shot with Doc Ock there is no station visible in the background. (01:33:00)
Continuity mistake: When Spider-Man takes back his suit from Jameson's office, Jameson looks up at the skyline, and there are no steel or metal bars running over the skyline, yet when the camera zooms out with Jameson yelling "I want Spider-Man", metal bars have appeared over the skyline that were not there in the previous shot. (01:29:55)
Continuity mistake: In the elevator scene when Peter/Spider-man first loses his powers, we see a man standing outside the elevator holding a dog on a leash. However, after Spider-man delivers the line about the costume riding up on the crotch, we see the man use both hands to adjust his jacket, and the leash is nowhere to be found, nor is their any indication that the dog is on the elevator. (00:29:30 - 00:30:35)
Continuity mistake: At the beginning of the film when we see Peter in Jameson's office, the black guy says, "Five minutes to deadline Jonah." Jameson raises his hand to him, but in the following shot it's a lot lower. Also, when he's talking about a food poisoning scare, he raises his hand high up to the right, yet in the following shot, it's far to the left of him. (00:07:45)
Continuity mistake: Just after the bank robbery, Doc Ock and Spider-Man are fighting on the side of the building. When some rocks fall down, there is a man (that looks like Stan Lee) pulling a girl away from falling debris. There is a red car to the right of the screen and a yellow taxi to the left, yet in the following shot from a different angle, there are a few more people in front of the red car, and no dust on the ground where the rocks hit the floor. (00:48:35)
Continuity mistake: During the scene where Peter is on his way to the play, he stops the cop car flying through the air by catching it in a web, but the glass shards and debris which are flying along with the car are not in the web anywhere, nor do they hit the people in the path of the car.
Continuity mistake: When Spider-Man is at the front of the train trying to stop it, the windows at the front are already smashed, yet when he spins a load of webs, the two panes at the front smash again. (01:34:35)
Continuity mistake: As Mary Jane is reunited with John Jameson after Spider-Man lowers her down from the crane, one shot shows tears along Spider-Man's suit along the left leg while he is crouching. However, in a later shot, he stands up to swing away and the tears are now running along his right leg.
Continuity mistake: At the very beginning when Peter walks into the pizza place with his boss, a policeman walks out, In the following shot, the policeman has vanished. (00:03:30)
Continuity mistake: After Peter came out of the deli, you could see and hear people running around screaming hysterically. But when the shot showed Peter's glasses falling to the ground in slow motion, you could see people were just walking down the street leisurely.