Audio problem: During the opening credits (while it says: "assistant director") you can briefly hear the next bit of music start 12 seconds too soon - there are a couple of notes from "three blind mice" starting to fade in from the percussion music. But more time was needed, so the last 12 second of the percussion number are repeated. A slightly clumsy edit to cover the credits running longer than planned (best heared with headphones).
Continuity mistake: When we first see Bond and Quarell talking in the bar, Quarell asks if Bond likes good eating, to which Bond replies he does. Camera cuts and his entire facial expression has changed from smiling and laughing to a deadpan serious one. (00:28:30)
Revealing mistake: Bond, Quarrel, and Honey are supposed to be walking through a saltwater river and yet none of them seem to react when coming out of the water with their eyes open so obviously it's fresh water used in the river. (01:15:10)
Suggested correction: Freshwater... river.
Other mistake: In Strangways' house, Bond picks up a framed picture. Just as the other person comes into the shot the image skips. (00:23:30)
Revealing mistake: The second car chase is made with the help from a greenscreen. The car's movements as seen on the background doesn't always correspond with Bond's steering and the following car is sometimes too close to the screen. (00:48:10)
Continuity mistake: When Honey is telling Bond that her shells could be sold for $50 in Miami, not only does the background appear to be the wrong section of the beach (it looks like the area up where her boat is stashed, not down on the sand bar) but at the bottom of the screen, some kind of buoy or flotation device bobs up on the water just behind Ursula Andress. A couple shots later, however, when they're running along the beach, the whole area is completely clear. (01:04:35)
Continuity mistake: During dinner at the table, when Dr. No crushes the Buddha with his hand, the grip changes positions between shots. (01:29:50)
Continuity mistake: When you see Bond in his hotel room for the first time, there are two bottles on the table, a clear one and a green one. When he re-enters the room and checks his traps, the green bottle has disappeared. Bond takes a bottle from a drawer but that also is clear so what happened to the green one? (00:23:40 - 00:41:00)
Other mistake: In the closing credits, John Kitzmiller's name is misspelt "John Kitzmuller". (01:45:50)
Continuity mistake: When Bond first meet Honey, in the first clip, when she's seen from the front, the sun is to her right (there is a bit of shadow on her left shoulder) but in the next clip, when she's seen from the back, the sun's to her left. (00:59:55)
Revealing mistake: Bond, Honey and Quarrel are hiding under the water when the guards arrive. They are not noticed, but one guard comes later than the others. Bond stabs him in the back and leaves him in the water, but there is no blood. (01:08:45)
Continuity mistake: When Dr. No crushes the gold Buddha statue at the dinner table with his hands, it falls off the table onto the floor. However, when a wider camera angle is shown, right before the guard takes Bond to the elevator, the gold statue is back on the table. (01:29:05 - 01:29:50)
Continuity mistake: At the baccarat table at the ambassador's club, Sylvia Trench has a red dress on. A piece of the dress off of her shoulder. In the first shot, this piece goes to the left about three inches from the broach she is wearing. In the next shot, the piece hangs down, covering half her broach. (00:07:00)
Continuity mistake: Following their repast, Dr. No instructs his minions to soften Bond up, which they do with great enthusiasm. When Bond regains consciousness in the cell, his brown Nehru jacket, while a little scuffed, is quite presentable. Shortly after, when he removes it to create protection for his bare hands, his undershirt is ripped severely, and there is a blood stain on it. (01:30:30)