Visible crew/equipment: When Michael asks Selene who started the war she answers "They did. Or at least that's what we are led to believe". If you look really close on the side of the window you can see the shadow of the boom mike move. (00:57:15)
Continuity mistake: When Selene runs up the stairs to release Michael from the chains, the door she left wide open is almost closed when the pursuers reach it. (01:08:55)
Revealing mistake: When Lucian is draining blood from Michael, the tip of the syringe bends like rubber. (01:21:25)
Continuity mistake: When Michael was captured and hung up, and meets Lucian face to face, Lucian injects blood into him and the syringe has an air bubble in it. In the next shot, the bubble has gone and the syringe is entirely filled with blood when being injected into Michael. (01:23:00)
Visible crew/equipment: During the final battle, a vampire shoots into a totally dark room and kills a werewolf. He then lifts his gun and people are seen reflected in the sights. (01:37:05)
Revealing mistake: After Selene bites Michael, Viktor picks him up and throws him through a wall. On the other side, he lands in a pool of water as do lumps of the wall, which float briefly before the shot ends. (01:43:55)
Revealing mistake: The edges of Selene's bright blue contact lenses are VERY visible in the scene near the end where she is on the ground, talking to Viktor. (01:44:30)
Continuity mistake: After Selene has killed Viktor she approaches Michael from behind. We see him turning his head. When it cuts he turns his head again. (01:46:10)
Revealing mistake: In the fight scene between Michael and Viktor, Viktor throws Michael in to a column and Michael lands face down. You can see his wire harness bulging under his pants. (01:47:15)
Revealing mistake: In the end fight scene between Viktor and the hybrid Michael, Viktor picks Michael up and throws him into a stone column. Terrible wirework then comes into play: Michael travels far too slowly towards the column for someone who was just thrown, he makes one perfect spin in the air on his way, and when he finally connects with the column, it's a delicate bounce off before the wire drops him on the ground. His legs don't even move from the "impact". (01:47:15)
Revealing mistake: At the end of the film when Viktor and Michael are fighting you can see Viktor pulling his punches. (01:47:35)
Continuity mistake: At the end when the 3 vampires are shooting at Michael, Selene drops behind them. The blood on the left side of her head disappears and reappears a couple of times. When she's taken them out it's back again. And it gets bigger and smaller all during the time it's there. (01:48:00)
Revealing mistake: When Selene notices the sword laying on the rocks, the sword moves up about two inches between shots, you can judge it by looking closely at the handle of the sword. (01:49:25)
Continuity mistake: During the fight scene towards the end, Michael Corvin (now half lichen/half vampire) flies through the air from being hit. At this point Michael’s color is a translucent black/grey. As he flies through the air you can see that his feet are white. (02:00:35)
Character mistake: On two occasions throughout the film, Kraven's accent changes - once when he is talking to Lucian near the beginning of the movie and near the end of the movie when he is telling Selene that Viktor killed her family.
Factual error: Every time the silver nitrate is shown on clothing, it is shown as silver. Silver nitrate in fact is black once exposed to light.
Plot hole: Even assuming that creating a manhole underneath your own feet Looney Tunes-style shooting a gazillion bullets around you through the floor is a better battle strategy than using said bullets to shoot at the three remaining wolves charging at you in the small corridor, said creatures don't suddenly stop existing just because you fell down one floor, making their complete disappearance - they do not give chase through the hole or stairs nor even make as much of an angry sound throughout the rest of the scene.
Suggested correction: I think this is more a stupidity than a plot hole. She could have escaped or defeated the wolves in any kind of ways, it's not a plot hole that she escaped by using a tactic that is illogical but not impossible.
I would absolutely agree about the silly tactic itself, but there were pursuing werewolves in that corridor, and for the remainder of the scene she just faces Lucian. There's no explanation why they don't come through the same hole, or take the stairs, or claw a hole through like they seemed to easily do in Michael's apartment.Not even a snarl: she drops one floor and they are...gone? So that part feels like a plot hole to me.
Continuity mistake: Right before the morphing shot of Michael's apartment building number, 510, we see Kraven enter and then leave the room through some double doors. As he enters, one of the doors is fully open and the other is half open. When he leaves they are both fully open.
Revealing mistake: In the fight scene between Michael and Viktor, you can see several times where Michael's dark colored make-up has been wiped off his lips.
Plot hole: Singe states that Viktor and Amelia are pure-blooded Vampires, and seems to agree when Viktor calls the story of the Corvinus Clan a legend. However, Evolution shows that Lucian had formed a partnership with Andreas Tanis, a Vampire who was outcast by Viktor specifically because he knew the truth of the Corvinus Clan and the origins of the Vampire and Lycan bloodlines. Given his lack of loyalty to the Vampires, there is no reason why Tanis would not have told Lucian that Viktor and Amelia are not pure-bloods, or that the legend of the Corvinus clan was entirely true. Lucian would have passed this information to Singe, as it would be vital to his work.