Trivia: This film's trailer was infamous for a line of dialogue (that didn't appear in the actual film) in which Cassie woodenly says, "He was in the Amazon with my mom when she was researching spiders, right before she died." John Mulaney sarcastically compared the line at the 2024 Oscars ceremony to memorable quotes from Jaws and When Harry Met Sally.
Trivia: During the final montage, when Cassandra says the line "standing up for what you believe," we see a shot of a camera swooping upwards towards a building's rooftop. The shot of the building is actually reused footage from the movie "Spider-Man 2," in a scene where Peter suits up and swings upwards in an alleyway. The only difference is that "Madame Web" sped up the shot ever so slightly.