Trivia: This film's trailer was infamous for a line of dialogue (that didn't appear in the actual film) in which Cassie woodenly says, "He was in the Amazon with my mom when she was researching spiders, right before she died." John Mulaney sarcastically compared the line at the 2024 Oscars ceremony to memorable quotes from Jaws and When Harry Met Sally.
Trivia: During the final montage, when Cassandra says the line "standing up for what you believe," we see a shot of a camera swooping upwards towards a building's rooftop. The shot of the building is actually reused footage from the movie "Spider-Man 2," in a scene where Peter suits up and swings upwards in an alleyway. The only difference is that "Madame Web" sped up the shot ever so slightly.
Trivia: The wallpaper in the hotel room, where the girls spend the night after the diner incident, matches the carpet in the hotel from The Shining.
Trivia: This film portrays Madame Web as a 30-year-old. In most versions of the comics, she is an old woman.
Trivia: While hosting an episode of Saturday Night Live, Sydney Sweeney poked fun at this film's box office performance.
Trivia: This is director S.J. Clarkson's film debut. She previously served as the director for many television series, including two Marvel properties in Jessica Jones and The Defenders.