Continuity mistake: When Peter and MJ hug on Tower Bridge he gets some blood from his face onto the left shoulder of her jacket. From other angles there's no blood.
Continuity mistake: In the (phony) bar, Peter's straw goes from bendy to straight to bendy to straight in different shots.
Continuity mistake: After Spidey fights with Beck for the first time he gets a wound on his right cheekbone which is fresh, then dry, then fresh again.
Audio problem: When Happy is talking to Peter in the kitchen at the charity event, from the angle over Happy's shoulder he's talking but the side of his face isn't moving, from that angle we should see his chin move up and down.
Continuity mistake: When Beck and Peter are talking together on top of a building, their heads change positions several times between shots. From looking at each other to straight ahead and every other direction.
Factual error: When the teacher announces they are going to Prague, they basically walk around the corner to a waiting bus. This is impossible because the part of Venice they are in is inaccessible to motor vehicles.
Factual error: The German woman screams "Nacht Monkey" when Peter jumps off the train, but the German for "Night Monkey" is "Nachtaffe".
Suggested correction: But his name is "Night Monkey" and would be called that in Germany so she simply confused her German with the English name, perfectly reasonable if she is afraid. Germans call Spider-Man the same as well, not "Spinnemensch."
Factual error: When Peter and Happy fly from the Netherlands to London, they pass the Dorset Coast, which is 150 miles southwest of London and therefore way off the direct route and a huge detour, even though they are supposed to be in a hurry. (01:32:50)