Continuity mistake: In the submarine dock of Geheimhaven, Captain Mohler is a man with dark hair in his mid or late thirties. But during the procession, he seems to be much older, with grey hair.
Factual error: There's absolutely no way Sallah and Indy would be able to lift that 'lid' (for lack of a better word) off the ark. That thing is solid rock and would weigh close to a metric ton - and none of them look like professional deadlifters to me.
Factual error: When Indy is attempting to steal the Ark when driving the truck, one of the soldiers shoots him in the arm. Then another German punches him repeatedly in the same arm, causing Indy great pain and injury. After suffering a wound like that there's no way he'd be able to move that arm, let alone raise it above his head. But the next day after the boat is stopped by the sub, Indy jumps into the water and swims to the sub without any problems.
Continuity mistake: When Indy stands inside the Map Room, looking at the model of the city, the rays of the sun are to his left, and they move right and down as the sun rises. But since the entry hole is behind him, that would mean that the sun would be to the right when standing outside - but it was to his left when he entered the hole.
Continuity mistake: When the temple collapses, Indy is holding the idol with his right hand against his chest, and the left hand free and arm extended. A frame later, both arms are extended and holding the idol.
Continuity mistake: When the temple collapses, Satipo is crouching looking at the chaos. Indy starts to run and there's a wide angle of the passage ahead but Satipo is nowhere around. He couldn't have managed to run that distance in half a second.
Suggested correction: He had a couple of seconds to run away from that spot.
Audio problem: When the camera focuses on Belloq as he looks into the Ark, Toht's screams are inaudible, despite being right next to the Frenchman. Only when the shot changes to show Toht his screams can be heard.
Continuity mistake: As Gobler's jeep is returning to the road after being hit by Indy, Gobler's goggles move from his eyes to his cap between shots.
Continuity mistake: When Dietrich and Toht look at the ghosts that pass behind them, the column of mist that is coming out of the Ark disappears.
Continuity mistake: Between the shots of Indy dressing as a Nazi and Captain Mohler reporting to Belloq and Dietrich, the Wurrfler appears to have advanced several inches forward (taking as reference the background behind the submarine, Mohler's location, and the fact that Belloq turns his head to the left to look at the Captain).
Continuity mistake: As Dietrich, Toht, Belloq and Marion board the Wurrfler, a naval officer descends the ladder and turns, his back to the ladder. In the next shot, he is facing the ladder.
Continuity mistake: In the Bantu Wind, the right arm of the soldier standing next to where Indy is hiding changes its position twice during Katanga's and Dietrich's speech.
Continuity mistake: The Angel of Death, judging from the position of the lid of the Ark (the two angels can be seen below the ghost), is located just above Dietrich's head. However, Toht screams as if it were right in front of him and Dietrich as if it were in front of Belloq (judging by their looks).
Continuity mistake: The distance between Otto and the Nepalese changes between shots, just as Toht begins saying "Surely he told you..."
Continuity mistake: In the shot after Belloq's death, the generator and the rock Indy and Marion are on disappear.
Continuity mistake: As Dietrich and Gobler come into the tent, Toht's leather case can be seen already opened. In the next shot, the case is suddenly closed.
Audio problem: After Dietrich pours liquor for himself, he speaks to Belloq. One of the words of his phrase (probably monsieur or Belloq) was cut from the audio as Dietrich's lips still move but nothing can be heard.
Visible crew/equipment: During Marion and Belloq's beverage contest, a round klieg lamp's blue light stands behind Belloq, which sometimes changes its intensity. Also, when Marion grabs the knife, behind her a moving light can be seen (like a helicopter's light), which disappears a few seconds later. This light also appears for a few seconds when Toht frees Marion's arm.
Continuity mistake: When Indy abruptly brakes the truck and the Ark box slides backwards, the poles on either side of the box disappear for the rest of the chase.
Audio problem: Just before Belloq opens the staff car's door, Paul Freeman's voice can be heard saying "Let's get out of here" but Belloq's lips don't move.