Revealing mistake: When the scientists inject the mouse with poison, they put it in a jar. The, after they place it down you can see someone's hand swap the jar for one containing a dead mouse. You can even hear it. (01:17:58)
Continuity mistake: When Ariadne wakes in the Snow Fortress and the floor goes from under her, we can see her gloves fall in to the crack but in a different camera shot of her we can see the gloves are now back near her head again. (02:12:20)
Continuity mistake: Just before the giant tarantula attacks the house where heroine Mara Corday is studying, you can see several book on the library table at the big picture window. The largest book on the table keeps shifting positions in several scenes.
Factual error: When TCAS sounds on an airliner, it should include instructions for the pilot to climb or descend. The pilot should not have to call another plane to inform that pilot. It would take too much time.
Character mistake: In the scene where Steve and Josie prepare to leave to fight the giant robot at the beginning of the movie, Steve tells Will "Oh, Will, a reminder: a lot of the kids at Sky High will only have one super hero parent, not two." When he says "one super hero parent", he holds up two fingers. When he says "not two", he holds up only one finger.
Other mistake: When McCroskey gets the information about Joe Seluchi, he's told that Seluchi purchased life insurance and was impotent. McCroskey informs Ted about Seluchi and the bomb he's carrying but nothing else. When Ted confronts Seluchi, he mentions both the insurance and the impotence.
Character mistake: When Harley asks Tony what the light is coming from his chest, Tony responds "an electromagnet." Harley replies "What does it power?" An electromagnet is not a source of power. The arc reactor is the power source which powers both the electromagnet (to keep Tony safe from the shrapnel near his heart) and the Iron Man suit. (00:43:25)
Other mistake: Towards the end of the movie, when Superman drops Gus off at the coal mine, Gus begins talking with the coal miners about him and Superman. He then rips open his shirt (as if he had a Superman costume on underneath). How did Gus know that Clark Kent tore his shirt like that in order to change into Superman, or keeps his suit under his clothes at all? The only people who know this fact are the ones who have seen the transformation from Clark to Superman, i.e. the audience.
Continuity mistake: In most of the scenes it is quite evident that Jessica has only a thin green dress on; when she meets Box she takes it off and she is nude. Later when she is outside of the dome and walking through the woods, she ducks to avoid some brush and she now has underwear on.
Continuity mistake: At the beginning after the car skids on the road and stops on the left side there are trees and snow but not the road, however the truck hits them on that side.
Factual error: In the diner, the kids listen to/dance to Britney Spears' "Toxic," which didn't release as a single until January 2004. This takes place in 2003.
Continuity mistake: At the start of the movie when the gun store is being robbed, one of them fires a portable rocket launcher at the police car, causing it to roll over and spin. As it comes to a stop just outside the store, it's visible that the driver's side window has been blown out, and the vehicle interior is visible, however when the same guy prepares to fire the bazooka to finish it off, the window has returned and is completely intact.
Factual error: When they first pick up the Mona Lisa, they show the back. There you can see a canvas sheet over a wooden framework. However, the Mona Lisa is painted directly onto wood, no canvas at all. The scan they run even says it's painted onto wood, despite visual evidence to the contrary. (00:06:35)
Continuity mistake: When Fin stops near the school bus, the bus number changes from "278146" to "278245" during the rescue of the kids.
Plot hole: Even if the white blood cells will attack and destroy the sub and the body of Doctor Michaels the atoms would still remain and take normal size after the critical 1 hour is up. This would also apply to the laser gun which they forgot or left behind on purpose, as well as several dozen litres of saline solution which were miniaturised and pumped into Benes' body. He's in a lot of trouble.
Visible crew/equipment: Throughout the movie, in the shots of the guys on the alien planet, the white reflector screens and film crew's reflections are visible in their sunglasses. (00:40:20)
Continuity mistake: Darth Maul's neck is black, but when he is talking to Darth Sidious on Coruscant, his neck is flesh-toned. (00:38:45)
Revealing mistake: Mr Hyde makes no bubbles when growling underwater. (01:11:30)
Continuity mistake: When Juni handcuffs the metal box to his wrist, it switches back forth from his right wrist to his left over and over again. (00:35:05)
Factual error: When the bus breaks down, steam is shown coming out of the front of the bus. GMC buses have the engine and radiator in the rear.