Continuity mistake: When Mario meets up with Luigi in the cockpit, the yellow eyes on Luigi's frog suit turn white. (00:10:12)
Revealing mistake: When Mario says that he must be having a nightmare, his air bubbles freeze for a few frames. (00:08:00)
Revealing mistake: When the force field blocking the pipe to the real world disappears, it remains for a short time. (00:09:47)
Revealing mistake: When the Doom Sub is warped to Desert Land, it remains for a short time. (00:10:29)
Continuity mistake: When Mario flies past King Koopa and Kootie Pie, Kootie Pie's yellow bracelets turn grey. (00:10:10)
Revealing mistake: When King Koopa tells Mario to give up, the Note Block Mario is on is floating above the ground. (00:10:02)
Continuity mistake: When King Koopa tells Kootie Pie to turn on the engine, the lever is yellow, but in the next shot, it turns brown. (00:09:58)
Revealing mistake: When King Koopa tells Kootie Pie to be patient, the camera zooms in on him, but the background does not adjust to the change of perspective. (00:09:04)
Revealing mistake: When the Chain Chomps start eating some poles, they are frozen for a few frames. (00:07:32)
Audio problem: When King Koopa says he is going to find the Mario Bros, his mouth moves before any dialogue comes out. (00:07:04)
Continuity mistake: When the Doom Sub is hitting the clotheslines, its roof disappears briefly. (00:05:56)
Other mistake: When Mario asks if Luigi feels how he feels, Luigi's eyelid is not coloured in. (00:03:57)
Revealing mistake: When the gondolier tells Mario that Venice is being attacked, he is briefly layered outside his gondola. Additionally, his statement is false, as the Doom Sub has not arrived in the city yet. (00:01:08)
Continuity mistake: When Mario and his friends run over to the gondolier, Toad's pants disappear. (00:00:59)
Continuity mistake: At the beginning of the episode, Mario's brown hair turns black, like Luigi's. (00:00:39)
Continuity mistake: When Mario and Luigi are looking at the submarine before they jump out of their gondola, Luigi's left glove goes from being present to absent.
Continuity mistake: When Mario and Luigi jump out of their gondola, Luigi's left glove goes from absent to present.