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Continuity mistake: When Luigi and Toad run through the halls, the Sledge Bros. shake the floor. Toad's purple shoes turn skin-color. This also happens when the Flame Chomps fall out of a window. (00:07:17 - 00:09:44)
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Revealing mistake: When King Koopa leaves to prepare himself for the ceremony, his right arm passes through a door. (00:08:09)
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Other mistake: When Mario and Princess Toadstool enter the dungeon, the hatch they enter through briefly appears closed, but they still enter it regardless. (00:04:16)
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Revealing mistake: When Princess Toadstool says that she would never sign her kingdom over to King Koopa, even with the threat of Bob-ombs, she is holding thin air. (00:05:21)
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Continuity mistake: After Mario says that he is surprised that King Koopa is surrendering, Toad's blue trousers turn brown. (00:01:01)
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Revealing mistake: After Toad jumps over a Sledge Bro, it briefly overlaps him. (00:09:14)
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Continuity mistake: When Mario looks at the picture of King Koopa, his brown hair turns black. This also happens when he saves Princess Toadstool. (00:03:02)
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Revealing mistake: When Mario saves Princess Toadstool from being crushed by the Thwomp, his plunger is layered over it. In the same shot, some of the debris is layered over Princess Toadstool. (00:03:23)
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Revealing mistake: When a Dry Bones lands in front of the door, it is layered over it and Princess Toadstool. (00:02:40)
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Revealing mistake: When the Koopas jump into a Warp Pipe, part of Kootie Pie is missing. (00:01:55)