Continuity mistake: When Ash approaches the bumpers, his bag disappears. This also happens before he points out that the Machoke and Machamp are the same. (00:09:38 - 00:11:16)
Continuity mistake: When Ash and his friends leave, the stripe on Ash's bag changes from being above the circle to below it. (00:20:50)
Continuity mistake: When Emily says that this is the third time Psyduck ran away, May's gloves disappear. (00:13:18)
Continuity mistake: When Meowth is keeping the net closed, Psyduck's cream-coloured feet turn white. In the same shot, Jessie speaks, but her mouth does not move. (00:10:43)
Continuity mistake: When the camera zooms out on Ash and his friends sliding down the hill, May's gloves turn completely blue. (00:09:14)
Continuity mistake: When Ash tells Phanpy to use Defense Curl, his glove disappears while his right sleeve changes from black to blue. (00:03:10)
Continuity mistake: After Ash and his friends say that three against one is unfair, Machoke's black briefs turn blue like its skin. This also happens when it uses Cross Chop. (00:02:36 - 00:11:58)
Answer: Yes it ran away because Layla hurt its feelings.