TedStixon

Continuity mistake: When Koopa is chewing out Iggy and Spike for forgetting about the rock, the amount of wax on his hands changes multiple times between cuts. For instance, the wax covering his left hand disappears in one wide shot, but is present in every other shot. Additionally, the amount of wax on his right hand changes in amount several times.

TedStixon

Continuity mistake: When Koopa tells Daisy that women "never forget the first time they get kissed by a lizard," his face starts to morph. It then cuts to the opposite angle, and suddenly, between cuts, his hand has appeared up by his face.

TedStixon

Continuity mistake: During the car chase, after Mario says "This is driving!" Luigi goes from facing forwards to facing sideways at Mario between cuts.

TedStixon

Continuity mistake: After Toad is turned into a Goomba, when Koopa is talking about his own evolution, Toad goes from being shackled to the chair to be unshackled and leaning forward instantly between cuts.

TedStixon

Continuity mistake: When Koopa is explaining the devo chamber to Mario and Luigi, we see a monitor showing an x-ray of Toad's de-evolution. When the camera cuts from the wide shot to the closeup, the monitor has suddenly gone back in the animation and replays the same segment again.

TedStixon

Continuity mistake: When Mario and Luigi are giving their names to the officer, at one point, another officer breaks a glass and we see shards of it landing on the book in front of the officer. A few shots later, most of it has vanished.

TedStixon

Continuity mistake: When Mario and Luigi are giving their names to the officer, there's a woman randomly rubbing her foot against his arm and shoulder throughout the scene. However, the way her foot is on him changes multiple times throughout the scene.

TedStixon

Continuity mistake: When Big Bertha picks up the old lady, she goes from holding her in front of her to holding her above her head instantly between cuts.

TedStixon

Continuity mistake: When Mario and Luigi are in the cave, Mario goes from Luigi's side to behind, to being on the other side between cuts. There's no point in time where either they (or their feet) aren't on camera, so it makes no sense.

TedStixon

Continuity mistake: When Daisy and Luigi are looking at the fossils, after Luigi says "You're beautiful," the distance between him and Daisy increases instantly as it cuts to a wide shot.

TedStixon

Continuity mistake: When Daisy is showing Luigi the fossils, as the camera cuts from the wide shot to the closeup (when Daisy says "Have a look!"), suddenly their heads go from facing up to facing down between cuts, as do their flashlights.

TedStixon

Continuity mistake: When Iggy and Spike round the corner and snatch Mario's girlfriend, the look on Iggy's face changes as it cuts from a close-up to a wide shot.

TedStixon

Continuity mistake: When Mario is watching Luigi trying to talk to Daisy after she gets off the phone, he is tapping the empty water bottle against his head. In the first shot, it's against his forehead, but in the next shot, it's suddenly down by his cheek.

TedStixon

Continuity mistake: When Daisy approaches Mario and Luigi after making a call at the phone booth, Mario goes from looking sideways at Luigi to looking down under his car's hood between cuts.

TedStixon

Continuity mistake: When Koopa is pushed into the chair in the Devo Chamber, suddenly a puddle of goop appears on the floor, and you can see that Koopa is sitting atop an empty uniform that wasn't there before. (There was a short, deleted scene where Koopa used the chamber to reduce a worker to primordial slime, leaving the puddle on the ground and the empty uniform on the chair. However, as the scene was cut, the puddle and uniform appear out of nowhere in the finished film.)

TedStixon