Trivia: In the Area 51 levels when the player is inside the lab, a women on the intercom is heard saying names such as Lieutenant Deal, Doctor Francis, Captain Santiago, Doctor Lovering and Nurse Robinson. Those names are a reference to the band The Pixies.
Trivia: In the Pelagic II level, the guards are wearing outfits similar to Mario because the game is published by Nintendo.
Trivia: In the Chicago level, there is a piece of swiss cheese hidden inside the toilet seat in the bathroom.
Trivia: After the end credits finish, a line appears saying "Perfect Dark is Forever."