Deliberate mistake: After Wolverine arrives at 3 Mile Island he approaches through a thick fog. Above him are a series of conveyor belts that are used at a coal power plant, not a nuclear plant such as 3 Mile Island. The belts are unique to coal mines and coal power plants, and would not be found at a nuclear power plant. The filmmakers obviously couldn't film on the site of a nuclear plant, so filmed on a coal plant to fill in.

X-Men Origins: Wolverine (2009)
1 deliberate mistake - chronological order
Directed by: Gavin Hood
Starring: Ryan Reynolds, Hugh Jackman, Liev Schreiber, Danny Huston, Dominic Monaghan
Revealing mistake: When the old couple who give Wolverine clothes are first driving towards their house, there is an aerial shot of them driving over a bridge. This is supposed to be set in Canada (drive on the right) but it was filmed in New Zealand (drive on the left). They are driving on the right but the arrow on the road is on the left side.
Question: Near the end, in the lab, Victor Creed tells Kayla that her powers of persuasion will not work on him. Why is that?
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Chosen answer: Her power works by manipulating parts of the mind. Since Victor and Logan both regenerate her power can't affect them because they essentially "heal/regenerate" the part she is manipulating. Granted Prof. X threatens Logan with this in the original movies, but he is much more powerful, or could have been bluffing.