Revealing mistake: In the Director's Cut ending when Audrey II eats Seymour, it spits out his glasses, and they land in front of the camera. They pulled this off by having a pair of glasses inside the plant's mouth that would be yanked out by a string, and another pair of glasses would be dropped in front of the camera. However, the first pair gets caught in the teeth and fall back inside the mouth, but the other pair lands on the ground anyway.

Little Shop of Horrors (1986)
1 revealing mistake
Directed by: Frank Oz
Starring: Steve Martin, John Candy, Rick Moranis, Vincent Gardenia, Ellen Greene, Levi Stubbs
Continuity mistake: When Seymour tries to kill the plant he pulls a gun, the gun is a six shooter, the plant grabs the gun and fires at the guy, then he fires some more, in total the plant fires 9 shots with a six shooter...
Seymour: It's true! I chopped him up. But I didn't kill him.
Trivia: To make the effects of Audrey II look convincing, the animatronic was usually shot at a lower frame-rate, such as 12 frames per second, and then played back at the standard 24fps. This sped-up the shots and made the animatronic's motions look more fluid and lifelike. In order to compensate, whenever a human actor would be in-shot with Audrey II, they would have to act in "slow motion" in order for their movement to look right at 24fps. (You can kinda tell in a few shots if you look closely).
Question: Near the end of the song "I am a dentist", is Orin saying "and a success" or "and I say sit"?
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Answer: He says "and a success". He's singing about his mother telling him he'd become a successful dentist.