Continuity mistake: In the scene where Chicken Little lends the alien baby, Kirby, his handkerchief, after Kirby blows his nose, it sticks to Chicken Little's hand. Chicken Little then wipes the hanky off his hand on to the grass. In the very next shot, the hanky has disappeared, and never shows up again.
Continuity mistake: When Chicken Little is trying to open the locker, he uses a rope. This rope just disappears when he gets himself into the locker.
Factual error: When Chicken Little is in his bedroom, after he scored during his baseball game, he looks into the spoon he is using as a microphone to see his reflection. He looks into the side that is curved away from you. When you look at that side of a spoon your reflection is upsidedown, but Chicken Little's was right side up.
Continuity mistake: When the baseball gets stuck on the bull's horn in the Baseball game it is on the right horn, but when he touches Chicken Little with the ball it is on the left horn.
Other mistake: When Mr. Woolensworth is giving attendance, he calls out Fish Out of Water's name, and there is a close-up of him. As the camera zooms out, Mr. Woolensworth is missing for a few frames, then suddenly appears out of thin air. (00:09:08)